Optimal methods for evaluating antimicrobial activities from plant extracts
Optimal methods for evaluating antimicrobial activities from plant extracts
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DOI：
10.1016/j.mimet.2010.11.008
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发表时间：
2011-02-01
影响因子：
2.2
通讯作者：
Ting, Kang Nee
中科院分区：
文献类型：
--
作者：
Othman, Mukhrizah;Loh, Hwei San;Ting, Kang Nee
在过去的几十年里，从植物中寻找抗菌剂一直是人们越来越感兴趣的事情。然而，由于缺乏标准化，特别是采用的抗微生物方法，许多这些研究产生的结果不能直接进行比较。许多研究人员提出，有必要建立具有一致结果的方法来评价植物提取物的抗菌活性。尽管如此，仍然有许多文献报道了描述不同方法的研究。这项研究的目的是找到最佳的方法，为研究植物提取物提供一致的定量抗菌结果。本研究使用了三种不同的琼脂扩散法(浇注平板扩散法(PPDD)、条纹平板扩散法(SPDD)和井内琼脂扩散法(WA))和一种肉汤扩散法(浊度法)。从两种植物中提取的提取物对两种细菌(大肠杆菌和金黄色葡萄球菌)进行了抑菌试验。在基于琼脂的化验中。PPDD产生的结果重复性最好。TB能够显示受试样品对包括低极性植物提取物在内的细菌生长动力学的抑制作用。我们建议，在评估植物粗提物的抗菌活性时，应同时使用琼脂(即PPDD)和肉汤两种方法。(C)2010爱思唯尔B.V.保留所有权利。
The search for antimicrobial agents from plants has been a growing interest in the last few decades. However, results generated from many of these studies cannot be directly compared due to the absence of standardization in particular antimicrobial methods employed. The need for established methods with consistent results for the evaluation of antimicrobial activities from plant extracts has been proposed by many researchers. Nevertheless, there are still many studies reported in the literature describing different methodologies. The aim of this study was to find optimal methods to give consistent quantitative antimicrobial results for studying plant extracts. Three different agar-based assays (pour plate disc diffusion (PPDD), streak plate disc diffusion (SPDD) and well-in agar (WA)) and one broth-based (turbidometric (TB)) assay were used in this study. Extracts from two plant species (Duabanga grandiflora and Acalypha wilkesiana) were tested on two bacterial species, namely Escherichia coli and Staphylococcus aureus. Amongst the agar-based assays. PPDD produced the most reproducible results. TB was able to show the inhibitory effects of the test samples on the growth kinetic of the bacteria including plant extracts with low polarity. We propose that both agar- (i.e PPDD) and broth-based assays should be employed when assessing the antimicrobial activity of plant crude extracts. (C) 2010 Elsevier B.V. All rights reserved.