Cloning of the heart fatty acid-binding protein (H-FABP) gene and its tissue-specific expression profile in the Lanzhou fat-tailed sheep, Ovis aries
Cloning of the heart fatty acid-binding protein (H-FABP) gene and its tissue-specific expression profile in the Lanzhou fat-tailed sheep, Ovis aries
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兰州大尾羊心脏脂肪酸结合蛋白（H-FABP）基因的克隆及其组织特异性表达谱
DOI：
10.1016/j.smallrumres.2012.12.016
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发表时间：
2013-05-01
影响因子：
1.8
通讯作者：
Yang, J. T.
中科院分区：
文献类型：
--
作者：
Bai, J. L.;Xu, H. W.;Yang, J. T.
心脏脂肪酸结合蛋白基因（h-fabp）在细胞内脂肪酸转运中起重要作用。H-fabp已被克隆并测序，被认为是评估猪、鸡和牛肉质性状的功能性候选基因。然而，关于绵羊中这种基因的研究报道很少。本研究利用RACE技术克隆了兰州肥尾羊h-fabp的全长cDNA。通过实时定量PCR检测其在骨骼肌、肝脏和4个脂肪库7个发育阶段的组织特异性表达谱。分别拼接425 bp的5’-RACE cDNA、231 bp的3’-RACE cDNA和177 bp的中间片段，得到全长748 bp的h-fabp cDNA （GenBank登录号JQ780322）。系统发育分析表明，兰州肥尾羊与山羊（Capra hircus）亲缘关系较近。在1月龄羊的骨骼肌中，h-fabp表达水平极低。3月龄和5月龄羊的表达量增加，7月龄羊的表达量达到峰值。在9个月和11个月大的绵羊中，基因表达水平与3个月和5个月大的绵羊中观察到的mRNA水平相似。在13月龄的绵羊中，h-fabp mRNA的表达水平与1月龄的绵羊相似。在绵羊不同发育阶段骨骼肌中h-fabp的表达模式表明，h-fabp的表达随年龄的增长而下降。h-fabp在肝脏中的表达模式也得到了类似的结论。h-fabp mRNA在各发育阶段的皮下（s.c.）和内脏（v.s.）库中的表达量都很低，且在这些库中的表达水平无显著差异。然而，当我们将七个发育阶段的数据加在一起时，h-fabp的表达水平最高的是靠近枯萎的s.c.W，其次是肾周的v.c.P，网膜的v.c.O和靠近尾部基部的s.c.T。这些发现为今后研究h-fabp在绵羊脂肪沉积和脂肪酸代谢调节中的作用提供了基础数据。(C) 2013 Elsevier B.V.版权所有
The heart fatty acid-binding protein gene (h-fabp) plays an important role in intracellular fatty acid transport. h-fabp had been cloned and sequenced and is considered a functional candidate gene for assessing the meat quality traits in pig, chicken, and cattle. However, there have only been few studies reported on this gene in sheep. In the present study, we cloned the full-length cDNA of h-fabp in the Lanzhou fat-tailed sheep using RACE technique. Its tissue specific expression profile in the skeletal muscle, liver and 4 fat depots at seven developmental stages was determined by real-time quantitative PCR. A 425 bp 5'-RACE cDNA, 231 bp 3'-RACE cDNA and 177 bp intermediate fragment were spliced to obtain a 748 bp full-length cDNA of h-fabp (GenBank accession number JQ780322). The phylogenetic analysis revealed that the Lanzhou fat-tailed sheep is more closely related to the goat, Capra hircus. In skeletal muscle, h-fabp was expressed at a very low level in one-month-old sheep. The expression level increased in three- and five-month-old sheep, and a peak expression level was observed in seven-month-old sheep. In nine- and eleven-month-old sheep, the gene expression level was similar to the mRNA level observed in three- and five-month-old sheep. In thirteen-month-old sheep, the h-fabp mRNA level was similar to the low expression level observed in one-month-old sheep. The expression pattern observed in the skeletal muscle at different developmental stages in sheep indicates that h-fabp expression declines with age. A similar conclusion on the h-fabp expression pattern in the liver was drawn from our findings in this study. The amount of h-fabp mRNA in the subcutaneous (s.c.) and visceral (v.s.) depots at each developmental stage was very low, and there was no significant difference in the expression levels among these depots. When we added the data from the seven developmental stages together, however, the highest h-fabp expression level was observed in the s.c. fat near the wither (s.c.W), followed by the v.c. perirenal (v.c.P), v.c. omental (v.c.O), and s.c. fat near the base of tail (s.c.T). These findings provide basic data that may be useful in future studies that investigate the role h-fabp plays in fat deposition and the regulation of fatty acid metabolism in sheep. (C) 2013 Elsevier B.V. All rights reserved.