Coordinate activation of a target gene by KDM1C histone demethylase and OTLD1 histone deubiquitinase in Arabidopsis
Coordinate activation of a target gene by KDM1C histone demethylase and OTLD1 histone deubiquitinase in Arabidopsis
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DOI：
10.1080/15592294.2019.1603982
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发表时间：
2019-04-22
期刊：
影响因子：
3.7
通讯作者：
Citovsky, Vitaly
中科院分区：
文献类型：
--
作者：
Keren, Ido;Lapidot, Moshe;Citovsky, Vitaly
组蛋白去泛素化酶和组蛋白赖氨酸去甲基化酶之间潜在的功能协调代表了转录结果的表观遗传控制的最少研究的方面之一。在这里，这个问题得到解决，使用拟南芥组蛋白修饰擦除器去泛素化酶OTLD 1和去甲基化酶KDM1C已知在植物细胞中相互作用。OTLD 1和KDM1C的功能获得和丧失突变体的表征表明，这两种酶与其靶基因AN3的启动子染色质相关联，并作为其表达的共激活剂发挥作用。这种转录结果是由H3K9抑制标记的去甲基化所支持的，推测是由KDM1C组蛋白去甲基化酶活性所支持的。KDM1C和OTLD 1与靶染色质的缔合是相互依赖的，使得在不存在KDM1C的情况下在AN3处未检测到OTLD 1，并且在不存在OTLD 1的情况下KDM1C显示不同的且非功能性的缔合模式。因此，OTLD 1和KDM1C可以相互串扰，在AN 3启动子染色质处组装功能性共激活因子复合物，并设定KDM1C对甲基化H3K9的特异性，以确定正确的转录结果。
Potential functional coordination between histone deubiquitinases and histone lysine demethylases represents one of the least studied aspects of epigenetic control of transcriptional outcomes. Here, this question was addressed using Arabidopsis histone modification erasers deubiquitinase OTLD1 and demethylase KDM1C known to interact with each other in plant cells. Characterization of gain- and loss-of-function mutants of OTLD1 and KDM1C showed that both enzymes associate with the promoter chromatin of their target gene AN3 and function as coactivators of its expression. This transcriptional outcome was underlain by demethylation of the H3K9 repression mark, presumably by the KDM1C histone demethylase activity. Association of KDM1C and OTLD1 with the target chromatin was interdependent such that OTLD1 was not detected at the AN3 in the absence of KDM1C and KDM1C displayed a different and non-functional pattern of association in the absence of OTLD1. Thus, OTLD1 and KDM1C may crosstalk with each other to assemble a functional coactivator complex at the AN3 promoter chromatin and set the KDM1C specificity for the methylated H3K9 to determine the correct transcriptional outcome.