Distinct thresholds for CD8 T cell activation lead to functional heterogeneity: CD8 T cell priming can occur. independently of cell division
Distinct thresholds for CD8 T cell activation lead to functional heterogeneity: CD8 T cell priming can occur. independently of cell division
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DOI：
10.4049/jimmunol.170.5.2442
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Schmitt-Verhulst, AM
中科院分区：
文献类型：
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作者：
Auphan-Anezin, N;Verdeil, G;Schmitt-Verhulst, AM
为了检查CD 8 T细胞功能异质性的基础，我们分析了对部分与完全激动剂Ag的应答。与APC的相互作用需要延长一段时间，以设置响应于部分与完全激动剂Ag的细胞分裂所需的阈值。细胞溶解功能的获得仅限于分裂的T细胞群体。相反，产生IFN-γ和表达一些活化标志物的承诺阈值似乎较低，并且与细胞分裂无关。事实上，我们表征了响应于部分激动剂刺激的T细胞群，其致力于产生IFN-γ，但未能分裂或分泌IL-2。重要的是，这种活化的未分裂的群体表现为“致敏”而不是“无反应”，表明1）CD 8 T细胞的致敏可能由不依赖于细胞分裂的次优刺激诱导，以及2）如先前报道的，与Ag的接触并不总是诱导初始CD 8 T细胞的完全分化程序。
To examine the bases for CD8 T cell functional heterogeneity, we analyzed responses to partial vs full agonist Ag. An extended period of interaction with APCs was required to set the threshold required for cell division in response to partial as compared with full agonist Ag. Acquisition of cytolytic function was restricted to the divided T cell population. In contrast, the threshold for commitment to produce IFN-gamma and express some activation markers appeared lower and independent of cell division. Indeed, we characterized a T cell population stimulated in response to the partial agonist that was committed to produce IFN-gamma, but failed to divide or secrete IL-2. Importantly, this activated nondivided population behaved as "primed" rather than "anergized," indicating 1) that priming of CD8 T cells may be induced by suboptimal stimulation independent of cell division and 2) that encounter with Ag does not always induce a complete differentiation program in naive CD8 T cells, as previously reported.