Frapbot: An open-source application for FRAP data
Frapbot: An open-source application for FRAP data
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DOI：
10.1002/cyto.a.23172
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发表时间：
2017-08-01
期刊：
影响因子：
3.7
通讯作者：
Schmidt, Samuel
中科院分区：
文献类型：
--
作者：
Kohze, Robin;Dieteren, Cindy E. J.;Schmidt, Samuel
我们介绍了Frapbot，一个免费的开源软件Web应用程序编写的R，它提供了手动和自动分析的荧光恢复后光漂白（FRAP）数据集。对于自动化操作，从包含图像内各个感兴趣区域的时间相关强度值的列的数据表开始，模式识别算法识别相关列并识别预漂白值的存在或不存在以及光漂白的时间点。使用各种用户可定义的拟合选项，以批量方式即时可视化显示所有上传文件的原始数据、残差、标准化和箱形图，这些图显示了所有上传文件的一半恢复时间（t（1/2））的分布。拟合结果以.zip文件形式提供，其中包含.csv格式的输出表。或者，用户可以手动控制前面描述的任何选项。(c)2017年国际细胞计数促进学会
We introduce Frapbot, a free-of-charge open source software web application written in R, which provides manual and automated analyses of fluorescence recovery after photobleaching (FRAP) datasets. For automated operation, starting from data tables containing columns of time-dependent intensity values for various regions of interests within the images, a pattern recognition algorithm recognizes the relevant columns and identifies the presence or absence of prebleach values and the time point of photobleaching. Raw data, residuals, normalization, and boxplots indicating the distribution of half times of recovery (t(1/2)) of all uploaded files are visualized instantly in a batch-wise manner using a variety of user-definable fitting options. The fitted results are provided as .zip file, which contains .csv formatted output tables. Alternatively, the user can manually control any of the options described earlier. (c) 2017 International Society for Advancement of Cytometry