The stimulation of Na,K,Cl cotransport and of system A for neutral amino acid transport is a mechanism for cell volume increase during the cell cycle
The stimulation of Na,K,Cl cotransport and of system A for neutral amino acid transport is a mechanism for cell volume increase during the cell cycle
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DOI：
10.1096/fasebj.10.8.8666170
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发表时间：
1996-06-01
期刊：
影响因子：
4.8
通讯作者：
Gazzola, GC
中科院分区：
文献类型：
--
作者：
Bussolati, O;Uggeri, J;Gazzola, GC
几年来已经知道，细胞增殖的触发与Na、K、Cl共转运和中性氨基酸转运系统A的活性增加有关。这些系统在高渗收缩细胞的体积恢复期间也得到增强。我们在这里证明，在NIH 3 T3细胞的细胞周期中，细胞体积的增加与钾和氨基酸的细胞含量增加有关。布美他尼延迟细胞周期进程并阻碍体积增加。非代谢类似物2-甲氨基异丁酸是系统A的特异性底物，可部分取代细胞周期中积累的天然氨基酸作为细胞内渗透剂。因此，建议刺激Na，K，CL共转运和系统A，在增殖细胞中观察到的，导致细胞体积的扩大，通过增强的细胞内积累的无机和有机渗透剂和并发的，prophetically义务摄取水。
It has been known for several years that the triggering of cell proliferation is associated with an increase of the activity of Na,K,CI cotransport and of transport system A for neutral amino acids. These systems are also enhanced during the volume recovery of hypertonically shrunk cells. We demonstrate here that during the cell cycle of NIH3T3 cells, an increase in cell volume is associated with an enhanced cell content of potassium and amino acids. Bumetanide delays cell cycle progression and hampers volume increase. The nonmetabolizable analog 2-methylamino-isobutpric acid, a specific substrate of system A, can partially substitute natural amino acids accumulated during the cell cycle as intracellular osmolytes. It is therefore proposed that the stimulation of Na,K,CL cotransport and of system A, observed in proliferating cells, causes an expansion of cell volume through an enhanced intracellular accumulation of both inorganic and organic osmolytes and the concurrent, osmotically obliged uptake of water.