Semaphorin 3F gene from human 3p21.3 suppresses tumor formation in nude mice.
Semaphorin 3F gene from human 3p21.3 suppresses tumor formation in nude mice.
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DOI：
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发表时间：
2002-05
期刊：
影响因子：
11.2
通讯作者：
R. Xiang;Albert R. Davalos;C. Hensel;Xiao-Jun Zhou;Chris Tse;S. Naylor
中科院分区：
文献类型：
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作者：
R. Xiang;Albert R. Davalos;C. Hensel;Xiao-Jun Zhou;Chris Tse;S. Naylor
人类染色体3p21.3上的杂合性缺失在许多肿瘤类型中经常发生。在之前的一项研究中，我们的实验室证明了来自3号染色体的80kb的P1克隆可以抑制小鼠纤维肉瘤细胞系A9的致瘤性。将该克隆上编码的两个基因信号素3F(SEMA3F)和N23导入小鼠A9细胞，检测其对体外和体内生长特性的影响。转染人SEMA3F基因后，裸鼠的致瘤性完全丧失，而转染人N23对裸鼠的致瘤性无影响。此外，SEMA3F还能阻断紫杉醇或阿霉素处理的A9细胞的凋亡。人卵巢癌细胞株HY的表达结果与A9细胞相似，但小细胞肺癌细胞株GLC45不受SEMA3F基因表达的影响。
Loss of heterozygosity on human chromosome 3p21.3 is a frequent occurrence in many tumor types. In a previous study, our laboratory demonstrated that an 80-kb P1 clone from chromosome 3 suppresses the tumorigenicity of the mouse fibrosarcoma cell line A9. Two cDNAs corresponding to genes encoded on this P1 clone, semaphorin 3F (SEMA3F) and N23, were tested for their effects on in vitro and in vivo growth characteristics after transfection into mouse A9 cells. Transfection of SEMA3F cDNA resulted in complete loss of tumorigenicity in nude mice, whereas transfection of N23 had no effect. Moreover, SEMA3F also functioned to block apoptosis of transfected A9 cells treated with Taxol or Adriamycin. The human ovarian adenocarcinoma cell line HEY showed a similar result as A9 cells, but the small cell lung cancer line GLC45 was unaffected by expression of SEMA3F.