Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly(L-lactide) composite
Biocompatibility, degradability, bioactivity and osteogenesis of mesoporous/macroporous scaffolds of mesoporous diopside/poly(L-lactide) composite
复制标题
介孔透辉石/聚丙交酯复合材料介孔/大孔支架的生物相容性、降解性、生物活性和成骨作用
DOI：
10.1098/rsif.2015.0507
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发表时间：
2015-10-06
影响因子：
3.9
通讯作者：
Wei, Jie
中科院分区：
文献类型：
--
作者：
Liu, Zhulin;Ji, Jiajin;Wei, Jie
采用溶液浇铸和颗粒浸出法制备了具有生物活性的介孔透辉石(m-DP)/聚L-丙交酯(PLLA)复合支架。结果表明，m-DP加入PLLA后，中孔/大孔支架的降解性和生物活性显著提高，且这种改善与m-DP的含量有关。此外，含有m-DP的支架具有中和酸性降解产物的能力，并在浸泡过程中防止溶液中的pH下降。此外，含有m-DP的支架还能促进MC3T3-E1细胞的附着、增殖和碱性磷酸酶活性，这也是m-DP含量依赖的。此外，组织学和免疫组织化学分析结果表明，含有m-DP的支架明显促进了新骨的形成，改善了材料在体内的降解情况，表明材料具有良好的生物相容性。结果表明，m-DP/PLLA复合材料的中孔/大孔支架具有良好的骨再生能力。
Bioactive mesoporous diopside (m-DP) and poly(L-lactide) (PLLA) composite scaffolds with mesoporous/macroporous structure were prepared by the solution-casting and particulate-leaching method. The results demonstrated that the degradability and bioactivity of the mesoporous/macroporous scaffolds were significantly improved by incorporating m-DP into PLLA, and that the improvement was m-DP content-dependent. In addition, the scaffolds containing m-DP showed the ability to neutralize acidic degradation products and prevent the pH from dropping in the solution during the soaking period. Moreover, the scaffolds containing m-DP enhanced attachment, proliferation and alkaline phosphatase activity of MC3T3-E1 cells, which were also m-DP content-dependent. Furthermore, the histological and immunohistochemical analysis results showed that the scaffolds with m-DP significantly promoted new bone formation and improved the materials degraded in vivo, indicating goodbiocompatibility. The results suggested that the mesoporous/macroporous scaffolds of the m-DP/PLLA composite with osteogenesis had a potential for bone regeneration.