Fetal development of vomeronasal system in the goat
Fetal development of vomeronasal system in the goat
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DOI：
10.1016/j.devbrainres.2004.01.003
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发表时间：
2004-04-19
期刊：
影响因子：
--
通讯作者：
Osada, T
中科院分区：
文献类型：
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作者：
Takigami, S;Wakabayashi, Y;Osada, T
我们之前的研究从形态学上揭示了成年山羊犁鼻（VN）系统与啮齿动物和负鼠的犁鼻系统不同，并且哺乳动物中至少存在两种类型的VN系统。然而，两种类型的 VN 系统的发育在个体发育上是否不同以及山羊 VN 系统何时建立仍不清楚。在这项研究中，我们从形态学角度观察了山羊副嗅球（AOB）和VN神经元的胎儿发育情况。在胎儿中，表达 Gi2 的 VN 末端终止于整个 AOB 的肾小球，并且在到达 AOB 的神经末梢中未检测到 Go 的免疫反应性。 AOB的层结构在妊娠后半期迅速发育。在VN器官(VNO)中，妊娠中期，VN神经元的树突暴露在VN管腔内，VN神经元的突起上存在散在的、薄的微绒毛。在树突的顶端部分，不存在明显的囊泡。然而，嗅觉标记蛋白（OMP）的免疫组织化学显示，出生前的VN感觉上皮（VSE）中存在一些带有OMP的VN神经元，尽管在成人VSE中检测到明显的免疫反应性。胎儿 VN 神经元似乎发育不全。这些结果表明山羊 VN 系统在个体发育上与啮齿动物和负鼠 VN 系统不同。并且出生前发育不全。山羊 VN 系统将在产后早期发育和成熟，类似于啮齿动物和 OPOSSUM VN 系统。 (C) 2004 Elsevier B.V. 保留所有权利。
Our previous study morphologically revealed that the adult goat vomeronasal (VN) system was different from the rodent and opossum one, and at least two types of VN systems exist in mammals. However, it remains unknown whether the developments in both types of VN systems are ontogenetically distinct and when the goat VN system is established. In this study, we morphologically observed the fetal development of the goat accessory olfactory bulb (AOB) and VN neuron. In the fetus, Gi2-expressing VN terminals terminated at glomeruli throughout the AOB, and no immunoreactivities for Go were detected in the nerve terminals reaching into AOB. The layer structure of AOB rapidly developed in the latter half of gestation. In the VN organ (VNO), at the middle stage of gestation, the dendritic processes of VN neuron were exposed in the VN lumen, and scattered and thin microvilli existed on the protrusion of the VN neuron. In the apical part of dendritic processes, no clear vesicle existed. However, the immunohistochemistry of an olfactory marker protein (OMP) revealed that a few VN neurons with OMP exist in VN sensory epithelium (VSE) before birth, although marked immunoreactivities were detected in adult VSE. Fetal VN neurons appeared to be underdeveloped. These results suggest that the goat VN system is ontogenetically distinct from the rodent and opossum VN systems. and is underdeveloped before birth. The goat VN system will develop and mature during the early postnatal period similar to the rodent and OPOSSUM VN systems. (C) 2004 Elsevier B.V. All rights reserved.