Modulation of dietary fat on the toxicological effects in thymus and spleen in BALB/c mice exposed to perfluorooctane sulfonate
Modulation of dietary fat on the toxicological effects in thymus and spleen in BALB/c mice exposed to perfluorooctane sulfonate
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膳食脂肪调节对暴露于全氟辛烷磺酸的 BALB/c 小鼠胸腺和脾脏的毒理学影响
DOI：
10.1016/j.toxlet.2011.04.029
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发表时间：
2011-07-28
影响因子：
3.5
通讯作者：
Jiang, Guibin
中科院分区：
文献类型：
--
作者：
Wang, Yu;Wang, Ling;Jiang, Guibin
全氟辛烷磺酸（PFOS）可导致啮齿动物免疫器官萎缩，但其作用机制尚未完全了解。在这项研究中，BALB/c小鼠喂食常规（RD）或高脂饮食（HFD）。然后，它们连续14天暴露于全氟辛烷磺酸（0、5和20毫克/千克/天）。在RD暴露组中，体重显著下降，免疫器官显示出相当大的萎缩。组织学分析表明，胸腺的皮髓质交界处难以区分，脾脏中观察到窦扩张。透射电子显微镜（TEM）结果显示，胸腺和脾脏中出现脂褐质颗粒和空泡。胸腺细胞凋亡增加。在HFD组中，所有这些现象都没有消除。电镜下免疫器官萎缩较严重。在20毫克/千克/天全氟辛烷磺酸高脂膳食组中，胸腺小叶中的脂肪细胞甚至更多。在所有暴露组中，胸腺和脾脏中增殖物激活受体-α和白细胞介素-1 β的表达上调。这些结果表明，全氟辛烷磺酸可能通过干扰脂质代谢间接攻击免疫器官，导致胸腺和脾脏共同衰老。这些数据可能有助于了解全氟辛烷磺酸如何影响免疫系统。(C)2011年由Elsevier爱尔兰有限公司出版。
Perfluorooctane sulfonate (PFOS) can cause atrophy of the immune organs in rodents, but the mechanism underlying this action is not completely understood. In this study, BALB/c mice were fed a regular (RD) or high-fat diet (HFD). They were then exposed to PFOS (0, 5, and 20 mg/kg/day) for 14 days. In the RD-exposure group, body weight significantly decreased and the immune organs showed considerable atrophy. Histopathological analyses showed that the corticomedullary junction of the thymus was indistinguishable, and sinus expansion in the spleen was observed. Transmission electron microscopy (TEM) results showed that lipofuscin granules and vacuoles appeared in the thymus and spleen. Increased apoptosis of thymocytes was observed. In the HFD group, all of these phenomena were not eliminated. More serious atrophy was seen in the immune organs under TEM. Even more adipocytes were in the lobules of the thymus in the HFD 20 mg/kg/day PFOS groups. Expression of the proliferator-activated receptor-alpha and interleukin-1 beta were upregulated in the thymus and spleen in all exposure groups. These results suggest that PFOS may indirectly attack the immune organs by interfering with lipid metabolism, leading to co-senescence of the thymus and spleen. These data may aid understanding of how PFOS affects the immune system. (C) 2011 Published by Elsevier Ireland Ltd.