ABSCISIC ACID-RESPONSIVE SEQUENCES FROM THE EM GENE OF WHEAT
ABSCISIC ACID-RESPONSIVE SEQUENCES FROM THE EM GENE OF WHEAT
复制标题
DOI：
10.2307/3868997
复制
发表时间：
1989-10-01
期刊：
影响因子：
11.6
通讯作者：
QUATRANO, RS
中科院分区：
文献类型：
--
作者：
MARCOTTE, WR;RUSSELL, SH;QUATRANO, RS
我们证明了一个含有β-葡萄糖醛酸苷酶(GUS)报告基因的嵌合基因与小麦脱落酸(ABA)调控的646碱基对5‘’片段(-554至+92)相连，并在转基因烟草中正确表达。我们只在成熟种子的胚胎中观察到高活性，而在ABA上培养的未成熟种子表现出增强的表达。使用水稻瞬变实验，我们发现了一个260碱基对的片段(-168到+92)，它解释了ABA特异性的GUS表达增加了15倍。在ABA存在下，一个50碱基对的序列(-152到-103)在两个方向上都与截短的花椰菜花叶病毒启动子(35S)融合，使GUS活性增加三倍。在35S启动子和GUS的ATG之间插入EM5‘-非翻译区(+6到+86)，在没有ABA的情况下，GUS活性增加10倍。这些结果提示了EM5‘’区的以下两个功能片段：一个是从-152到-103的ABA反应元件，另一个是在+6到+86之间的元件，它可以定量地增加ABA反应。
We demonstrate that a chimeric gene containing the .beta.-glucuronidase (GUS) reporter gene linked to a 646-base pair 5'' fragment (-554 to +92) from the abscisic acid (ABA)-regulated from wheat is correctly expressed in transgenic tobacco. We observe high activity only in embryos of mature seeds, and immature seeds cultured on ABA show enhanced expression. Using a rice transient assay, we identify a 260-base pair fragment (-168 to +92) that accounts for the ABA-specific 15-fold increase in GUS expression. A 50-base pair sequence (-152 to -103) fused 5'' in either orientation to a truncated cauliflower mosaic virus promoter (35S) increases GUS activity threefold in the presence of ABA. Insertion of the Em 5''-untranslated region (+6 to +86) between the 35S promoter and the ATG of GUS results in a 10-fold increase in GUS activity in the absence of ABA. These results suggest the following two functional fragments of the Em 5'' region: an ABA response element from -152 to -103 and an element between +6 and +86 that quantitatively increases the ABA response.