Compact amperometric algal biosensors for the evaluation of water toxicity
Compact amperometric algal biosensors for the evaluation of water toxicity
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DOI：
10.1016/j.aca.2004.09.073
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发表时间：
2005-02
影响因子：
6.2
通讯作者：
Isao Shitanda;K. Takada;Y. Sakai;T. Tatsuma
中科院分区：
文献类型：
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作者：
Isao Shitanda;K. Takada;Y. Sakai;T. Tatsuma
以海藻酸钠凝胶或聚离子复合膜为载体，将单细胞小球藻直接固定在透明氧化铟锡电极表面。固定化藻类的光合产生的氧气进行监测安培。以6-氯-N-乙基-N-异丙基-1，3，5-三嗪-2，4-二胺（莠去津）、3-（3，4-二氯苯基）-1，1-二乙基脲（DCMU）、甲苯和苯4种有毒化合物作为还原电流的抑制率，评价了该生物传感器的响应。对阿特拉津、DCMU、甲苯和苯产生50%氧还原电流抑制的浓度（[公式：见正文]）分别为2.0、0.05、1550和3000μmoldm−3。这些数据与常规标准生长试验的数据之间具有良好的相关性。与传统的基于Clark型氧电极的藻类生物传感器相比，该传感器体积小，成本低，检测时间短（≤ 200 s）。
Unicellular microalga Chlorella vulgaris was entrapped in an alginate gel or a polyion complex membrane immobilized directly on the surface of a transparent indium tin oxide electrode. Photosynthetically generated oxygen of the immobilized algae was monitored amperometically. Responses of the algal biosensor to four toxic compounds, 6-chloro-N-ethyl-N-isopropyl-1,3,5-triazine-2,4-diamine (atrazine), 3-(3,4-dichlorophenyl)-1,1-diethylurea (DCMU), toluene and benzene, were evaluated as inhibition ratios of the reduction current. The concentrations that give 50% inhibition of the oxygen reduction current ( [Formula: see text] ) for atrazine, DCMU, toluene and benzene were 2.0, 0.05, 1550 and 3000μmoldm−3, respectively. There was a good correlation between these data and those of the conventional standard growth test. In comparison with the conventional algal biosensors based on the Clark-type oxygen electrode, the present sensor is much smaller and less expensive, and its assay time is much shorter (≤200s).