Functional and metabolic characterization of hemocytes of the green mussel, Perna viridis: in vitro impacts of temperature
Functional and metabolic characterization of hemocytes of the green mussel, Perna viridis: in vitro impacts of temperature
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DOI：
10.1016/j.fsi.2011.07.018
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发表时间：
2011-12-01
影响因子：
4.7
通讯作者：
Volety, Aswani K.
中科院分区：
文献类型：
--
作者：
Donaghy, Ludovic;Volety, Aswani K.
绿色贻贝，佩尔纳，是一种原产于亚洲的双壳类软体动物，最近被引入美国佛罗里达。自1999年在佛罗里达的坦帕湾首次观察以来，绿色贻贝的数量已大大增加，到达佛罗里达、格鲁吉亚和南卡罗来纳州的大西洋沿岸。目前关于绿色贻贝生态学和生物学的研究大多在印度洋和太平洋进行。最近，有人提出，由于低温抵抗力弱，绿色贻贝可能已经到达了它们在美国分布的北方边缘。然而，目前有一个明显的缺乏数据的适应能力的佩尔纳viridis在佛罗里达的环境条件，特别是在生理和细胞水平。在目前的工作中，我们确定和特点的人口循环血细胞，和细胞成分的血淋巴参与各种生理功能，包括免疫。两个主要群体的特点，透明细胞和粒细胞。粒细胞占循环细胞的60%，并显示出较高的吞噬能力，溶酶体含量和基础氧化代谢比透明细胞。血细胞参数不受绿色贻贝大小的影响。此外，对血细胞进行急性温度挑战（10、20和30摄氏度），并分析其免疫相关功能和代谢。我们的研究结果表明，10摄氏度代表了佛罗里达州的绿色贻贝的压力条件下，所描绘的低吞噬能力和氧化代谢的增加。(C)2011爱思唯尔有限公司保留所有权利。
The green mussel, Perna viridis, is a bivalve mollusk native to Asia and was recently introduced to Florida, USA. Since its first observation in 1999 in Tampa Bay, Florida, green mussel population has expanded considerably, to reach the Atlantic coast of Florida, Georgia and South Carolina. Most of currently available studies about the ecology and biology of green mussels were performed in the Indian and Pacific oceans. Very recently, it has been suggested that due to a weak low temperature resistance, green mussels might have already reached the Northern edge of their distribution in the USA. However, there is currently an obvious lack of data about the adaptation capacities of Perna viridis to environmental conditions in Florida, especially at the physiological and cellular levels. In the present work, we determined and characterized the populations of circulating hemocytes, and the cellular components of hemolymph involved in various physiological functions, including immunity. Two main populations were characterized, hyalinocytes and granulocytes. Granulocytes accounted for 60% of circulating cells, and displayed higher phagocytic capacities, lysosomal content and basal oxidative metabolism than hyalinocytes. Hemocyte parameters were not influenced by the size of green mussels. In addition, hemocytes were subjected to acute temperature challenges (10, 20 and 30 degrees C) and their immune-related functions and metabolism analyzed. Our results showed that 10 degrees C represent a stressful condition for the Floridian green mussels, as depicted by a low phagocytosis capacity and an increase of oxidative metabolism. (C) 2011 Elsevier Ltd. All rights reserved.