The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.
The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.
复制标题
DOI：
10.1016/j.ydbio.2007.06.020
复制
发表时间：
2007-09-01
影响因子：
2.7
通讯作者：
Zernicka-Goetz, Magdalena
中科院分区：
文献类型：
--
作者：
Torres-Padilla, Maria-Elena;Richardson, Lucy;Kolasinska, Paulina;Meilhac, Sigolene M.;Luetke-Eversloh, Merlin Verena;Zernicka-Goetz, Magdalena
在小鼠胚胎中，前-后轴的发育被认为只有在前内脏内胚层（AVE）出现并开始其不对称迁移时才开始。然而，最近发现AVE的标志物Lefty 1的表达在胚胎着床前开始。这一发现提出了两个重要的问题：显示这种AVE标记物的早期植入前表达的细胞是AVE的真实的前体吗？如果是，这如何有助于AVE的建立？在这里，我们解决这两个问题。首先，我们表明，另一个AVE标志物，Cer 1的表达，也开始植入前，其表达成为巩固的ICM细胞，包括原始内胚层的子集。第二，为了确定是否显示这种早期Cer 1表达的细胞是真正的前体的AVE，我们建立了条件，以跟踪这些细胞的延时研究，从早期围着床期阶段，直到AVE出现，并成为不对称位移。我们发现Cer 1表达细胞在植入后不对称地定位，随着胚胎的生长，它们分散成两个或三个簇。Cer 1在近端区域的表达逐渐减少，而在远端-外侧区域则增强。我们的时间推移研究表明，这一远侧域被纳入AVE与细胞中，Cer 1的表达开始后，才植入。因此，AVE由Cer 1表达细胞的祖先群体的一部分和稍后获得Cer 1表达的细胞形成。最后，我们证明，当AVE转移不对称建立前极，这发生在该地区的早期植入后表达Cer 1是最强的。总之，这些结果表明，在AVE迁移之前，已经预期到前后轴的方向。
Initiation of the development of the anterior-posterior axis in the mouse embryo has been thought to take place only when the anterior visceral endoderm (AVE) emerges and starts its asymmetric migration. However, expression of Lefty1, a marker of the AVE, was recently found to initiate before embryo implantation. This finding has raised two important questions: are the cells that show such early, pre-implantation expression of this AVE marker the real precursors of the AVE and, if so, how does this contribute to the establishment of the AVE? Here, we address both of these questions. First, we show that the expression of another AVE marker, Cer1, also commences before implantation and its expression becomes consolidated in the subset of ICM cells that comprise the primitive endoderm. Second, to determine whether the cells showing this early Cer1 expression are true precursors of the AVE, we set up conditions to trace these cells in time-lapse studies from early peri-implantation stages until the AVE emerges and becomes asymmetrically displaced. We found that Cer1-expressing cells are asymmetrically located after implantation and, as the embryo grows, they become dispersed into two or three clusters. The expression of Cer1 in the proximal domain is progressively diminished, while it is reinforced in the distal-lateral domain. Our time-lapse studies demonstrate that this distal-lateral domain is incorporated into the AVE together with cells in which Cer1 expression begins only after implantation. Thus, the AVE is formed from both part of an ancestral population of Cerl-expressing cells and cells that acquire Cer1 expression later. Finally, we demonstrate that when the AVE shifts asymmetrically to establish the anterior pole, this occurs towards the region where the earlier postimplantation expression of Cer1 was strongest. Together, these results suggest that the orientation of the anterior-posterior axis is already anticipated before AVE migration.
登录
查看更多内容
影响因子：
9.2
作者：
Perea-Gomez, A;Camus, A;Collignon, J
通讯作者：
Collignon, J
影响因子：
2.6
作者：
Richardson, Lucy;Torres-Padilla, Maria-Elena;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena
影响因子：
64.8
作者：
Brennan, J;Lu, CC;Robertson, EJ
通讯作者：
Robertson, EJ
影响因子：
4.6
作者：
Mesnard, Daniel;Guzman-Ayala, Marcela;Constam, Daniel B.
通讯作者：
Constam, Daniel B.
RNAi在小鼠胚胎植入植入术发展中信号通路的功能研究。
影响因子：
--
作者：
Soares, ML;Haraguchi, S;Torres-Padilla, ME;Kalmar, T;Carpenter, L;Bell, G;Morrison, A;Ring, CJA;Clarke, NJ;Glover, DM;Zernicka-Goetz, M
通讯作者：
Zernicka-Goetz, M