Evaluation of PCR to detect Theileria parva in field-collected tick and bovine samples in Tanzania
Evaluation of PCR to detect Theileria parva in field-collected tick and bovine samples in Tanzania
复制标题
DOI：
10.1016/s0304-4017(02)00448-x
复制
发表时间：
2003-03-10
影响因子：
2.6
通讯作者：
Bryant, M
中科院分区：
文献类型：
--
作者：
Ogden, NH;Gwakisa, P;Bryant, M
PCR检测感染的泰勒虫parva，东海岸热的原因，在现场收集的蜱和牛样本从坦桑尼亚的能力进行了评价。PCR检测到的感染率高（15/20，75%），在未进食的成年蜱蜱作为急性感染的小牛，但低（22/836，2.6%），未进食的成年蜱。从坦桑尼亚的野外采集的apapellulatus。蜱感染的患病率是在以前的研究中，使用唾液腺染色，以检测在现场收集的主机寻求蜱小锥虫感染。在282头自然暴露的瘤牛中，7头在检测泰勒虫属之前具有PCR阳性血沉棕黄层样品。寄生虫在染色Huffy外套细胞或淋巴结活检。泰勒虫的证据在109头小牛（38.6%）的淋巴结活检染色涂片和81头小牛（28.7%）的蓬松皮毛样本中检测到感染，而66头小牛（23.4%）的蓬松皮毛样本PCR呈T parva阳性。这些研究结果的PCR的灵敏度和特异性的影响进行了讨论。(C)2003 Elsevier Science B. V.保留所有权利。
The ability of PCR to detect infections of Theileria parva, the cause of East Coast Fever, in field-collected tick and bovine samples from Tanzania was evaluated. PCR-detected infection prevalence was high (15/20, 75%) in unfed adult Rhipicephalus appendiculatus ticks that fed as nymphs on an acutely-infected calf, but low (22/836, 2.6%) in unfed adult R. appendiculatus collected from field sites in Tanzania. Tick infection prevalence was comparable to that in previous studies that used salivary gland staining to detect T parva infection in field-collected host-seeking ticks. Of 282 naturally-exposed zebu calves, seven had PCR-positive buffy coat samples prior to detection of Theileria spp. parasites in stained huffy coat cells or lymph node biopsies. Evidence of Theileria spp. infections was detected in stained smears of lymph node biopsies from 109 calves (38.6%) and huffy coat samples from 81 (28.7%), while huffy coat samples from 66 (23.4%) were PCR-positive for T parva. Implications of these findings for the sensitivity and specificity of the PCR are discussed. (C) 2003 Elsevier Science B.V. All rights reserved.