MOLECULAR-CLONING AND EXPRESSION OF THE FAS LIGAND, A NOVEL MEMBER OF THE TUMOR-NECROSIS-FACTOR FAMILY
MOLECULAR-CLONING AND EXPRESSION OF THE FAS LIGAND, A NOVEL MEMBER OF THE TUMOR-NECROSIS-FACTOR FAMILY
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DOI：
10.1016/0092-8674(93)90326-l
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发表时间：
1993-12-17
期刊：
影响因子：
64.5
通讯作者：
NAGATA, S
中科院分区：
文献类型：
--
作者：
SUDA, T;TAKAHASHI, T;NAGATA, S
Fas抗原（Fas）属于肿瘤坏死因子（TNF）/神经生长因子受体家族，介导细胞凋亡。使用通过与人免疫球蛋白 Fc 融合制备的可溶形式的小鼠 Fas，在细胞毒性 T 细胞杂交瘤 PC60-d10S 的细胞表面上检测到 Fas 配体。使用荧光激活细胞分选仪分选高表达Fas配体的细胞群，并通过表达克隆从分选的细胞中分离其cDNA。氨基酸序列表明Fas配体是属于TNF家族的II型跨膜蛋白。 COS 细胞中表达的重组 Fas 配体诱导表达 Fas 的靶细胞凋亡。 Northern 杂交揭示 Fas 配体在活化的脾细胞和胸腺细胞中表达，这与其参与 T 细胞介导的细胞毒性以及在几种非淋巴组织（如睾丸）中的表达一致。
The Fas antigen (Fas) belongs to the tumor necrosis factor (TNF)/nerve growth factor receptor family, and it mediates apoptosis. Using a soluble form of mouse Fas, prepared by fusion with human immunoglobulin Fc, Fas ligand was detected on the cell surface of a cytotoxic T cell hybridoma, PC60-d10S. A cell population that highly expresses Fas ligand was sorted using a fluorescence-activated cell sorter, and its cDNA was isolated from the sorted cells by expression cloning. The amino acid sequence indicated that Fas ligand is a type II transmembrane protein that belongs to the TNF family. The recombinant Fas ligand expressed in COS cells induced apoptosis in Fas-expressing target cells. Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis.