Expression of hepatocyte growth factor mRNA in regenerating rat liver after partial hepatectomy.
Expression of hepatocyte growth factor mRNA in regenerating rat liver after partial hepatectomy.
复制标题
DOI：
10.1016/0006-291x(91)92020-k
复制
发表时间：
1991-05
影响因子：
3.1
通讯作者：
R. Zarnegar;M. Defrances;D. P. Kost;P. Lindroos;G. Michalopoulos
中科院分区：
文献类型：
--
作者：
R. Zarnegar;M. Defrances;D. P. Kost;P. Lindroos;G. Michalopoulos
肝细胞生长因子（HGF）是体外原代培养肝细胞的一种有效的完全有丝分裂原。有强有力的证据表明，这种新的生长因子可能介导肝细胞损伤后的再生。我们以前已经证明，大鼠部分肝切除或CCl4给药后不久，血清中免疫反应性HGF的量显著增加。在本文中，我们证明，肝切除术后3至6小时，大鼠肝脏中的HGF mRNA水平也急剧增加，在12小时达到峰值，并逐渐恢复到不可检测的水平，肝切除术后72至96小时。在单独的实验中，在部分肝切除术后的大鼠肝残留物中测定DNA合成（体内）。肝切除术后24小时DNA合成达到高峰，HGF mRNA表达高峰后12小时。这些结果表明，肝细胞生长因子可能是在肝再生过程中触发肝细胞增殖的主要早期信号之一。
Hepatocyte Growth Factor (HGF) is a potent complete mitogen for primary cultures of hepatocytesin vitro. There is strong evidence that this novel growth factor may mediate hepatocyte regeneration after liver damage. We have shown previously that the amount of immunoreactive HGF markedly increases in the serum of rats soon after partial hepatectomy or CCl4administration. In the present paper, we demonstrate that the level of HGF mRNA in rat liver also dramatically increases from 3 to 6 hours post hepatectomy, peaks at 12 hr and gradually returns to undetectable levels by 72 to 96 hours post hepatectomy. In separate experiments, DNA synthesis (in vivo) was determined in rat liver remnants after partial hepatectomy. DNA synthesis peaked 24 hr after hepatectomy, 12 hr after the peak of HGF mRNA expression. These results suggest that HGF may be one of the major early signals that triggers hepatocyte proliferation during liver regeneration.