Genome-wide identification and characterization of SRO gene family in wheat: Molecular evolution and expression profiles during different stresses
Genome-wide identification and characterization of SRO gene family in wheat: Molecular evolution and expression profiles during different stresses
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DOI：
10.1016/j.plaphy.2020.07.006
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发表时间：
2020-09-01
影响因子：
6.5
通讯作者：
Yin, Junliang
中科院分区：
文献类型：
--
作者：
Jiang, Wenqiang;Geng, Yuepan;Yin, Junliang
SRO（Similar to RCD ONE）是一类植物特有的小分子蛋白家族，在植物的生长发育以及对生物/非生物胁迫的应答中起着重要作用。虽然SRO的表征已经在模式植物中进行，但对它们在小麦中的功能，特别是在胁迫条件下的功能知之甚少。本研究从小麦基因组中鉴定了30个SRO基因（TaSRO）。它们在遗传学上分为两组，具有不同的结构。对TaSROs启动子区的顺式调控元件进行了分析，并对许多与胁迫应答和激素相关的功能元件进行了解释，揭示了TaSROs在小麦非生物胁迫和生物胁迫下诱导表达的原因。分析了小麦和其他7个物种（拟南芥、水稻、玉米、大麦、大豆、陆地棉和黄瓜）的SRO基因家族中的全基因组复制事件，分别产生1、12、9、23、6、5和3个基因对。组织特异性表达谱分析表明，TaSROs在小麦的一个或多个组织中高表达，可能在小麦的生长发育中起重要作用。此外，qRT-PCR结果进一步证实了这些TaSRO基因参与了小麦的逆境响应。综上所述，本研究为TaSROs的分子功能研究奠定了理论基础，尤其是在植物激素和生物/非生物胁迫响应中的作用。
SRO (SIMILAR TO RCD ONE), a type of plant-specific small protein family, play important roles in plant growth and development, as well as in response to biotic/abiotic stresses. Although characterization of SROs have been performed in model plants, little is known about their function in wheat, especially under stress conditions. In this study, 30 SRO genes were identified from the wheat genome (TaSRO). They were phylogenetically separated into two groups with distinct structures. The cis-regulatory elements in the promoter region of TaSROs were analyzed and numerous elements functionally associated with stress responding and hormones were interpreted, implying the reason for induction expression patterns of TaSROs during abiotic and biotic stresses in wheat. Whole-genome replication events in the SRO gene family of wheat and seven other species (Arabidopsis thaliana, rice, maize, barley, soybean, upland cotton, and cucumber) were analyzed, resulting in 1, 12, 9, 23, 6, 5, and 3 of gene pairs, respectively. The tissue-specific expression pattern profiling revealed that most TaSROs are highly expressed in one or more tissues and may play an important role in wheat growth and development. In addition, qRT-PCR results further confirmed that these TaSRO genes are involved in wheat stress response. In summary, our study laid a theoretical basis for molecular function deciphering of TaSROs, especially in plant hormones and biotic/abiotic stress responses.