Genetic and physiological evidence for the role of gibberellic acid in the germination of dormant Avena fatua seeds
Genetic and physiological evidence for the role of gibberellic acid in the germination of dormant Avena fatua seeds
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DOI：
10.1093/jexbot/49.318.89
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发表时间：
1998-01-01
影响因子：
6.9
通讯作者：
Foley, ME
中科院分区：
文献类型：
--
作者：
Fennimore, SA;Foley, ME
遗传和生理数据表明，赤霉素酸在野生燕麦种子休眠的调控中并不起主要作用。在40℃条件下，将完整的休眠种子分别成熟0、2、4、8、12、16和20周，分别在水、100 nM或1 μ M的赤霉素酸中吸收。催熟间隔的长度与平均碱基赤霉素浓度（萌发率为50%的浓度）成反比。因此，调控野生燕麦种子休眠释放的主要因素不是赤霉素酸，而是催熟后。F-2颖花（休眠x非休眠）根据萌发对逐渐升高的赤霉素浓度的响应进行分类，根据赤霉素需求：低、中、高进行汇总。将低、中、高赤霉素需要量池中F-3子代的萌发响应回归到F-2亲本值，计算赤霉素对萌发响应的遗传力h(2)=0.24。用200个十聚体引物对高、低赤霉素浓度F-2池DNA样本进行随机扩增多态性分析，未发现多态性。本研究结果表明，赤霉素酸不是野生燕麦种子休眠的主要调节因子。
Genetic and physiological data indicate that gibberellic acid does not have a primary role in the regulation of seed dormancy in wild oat (Avena fatua L.). The gibberellic acid sensitivity threshold of dormant caryopses imbibed for 7 d was 1 mu M. Intact dormant seeds were after-ripened for 0, 2, 4, 8, 12, 16, and 20 weeks at 40 degrees C and imbibed in H2O, 100 nM or 1 mu M gibberellic acid. The length of the after-ripening interval was inversely related to the mean base gibberellic acid concentration (the concentration resulting in 50% germination). Thus, after-ripening, not gibberellic acid, is the principal factor that regulates the release of seed dormancy in wild oat. F-2 caryopses (dormant x non-dormant) classified by germination response to progressively higher gibberellic acid concentrations, were pooled according to their gibberellic acid requirement: low, medium and high. Germination responses of the F-3 progeny from the low, medium, and high gibberellic acid requirement pools were regressed on to the F-2 parent values, and a heritability for germination response to gibberellic acid, h(2)=0.24, was calculated. Random amplified polymorphic DNA analysis of DNA samples from F-2 pools requiring low and high gibberellic acid concentrations were screened with 200 decamer primers and no polymorphisms were found. The findings of this investigation demonstrate that gibberellic acid is not the primary regulator of seed dormancy in wild oat.