Autoradiographic analysis of monoclonal antibody distribution in human colon and breast tumor xenografts
Autoradiographic analysis of monoclonal antibody distribution in human colon and breast tumor xenografts
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人结肠和乳腺肿瘤异种移植物中单克隆抗体分布的放射自显影分析
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
H. Sands
中科院分区：
文献类型：
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作者：
P. Jones;B. Gallagher;H. Sands
通过分析识别不同肿瘤相关抗原的两种放射性碘标记的单克隆抗体B6.2和B72.3的细胞分布，研究了单克隆抗体对裸鼠中人肿瘤异种移植物的靶向作用。i.在Clouser人乳腺癌（B6.2阳性，B72.3阴性）和LS 174 T人结肠直肠癌（B6.2阳性，B72.3阳性）中每种抗体分布的时间过程。使用放射自显影技术比较V.注射，所述放射自显影技术也用于测定与放射性标记的抗体体外孵育后的结合模式。125 I-B72.3在LS 174 T中的体内和体外定位的特征在于抗体与富含抗原的粘蛋白球的结合。相反，B6.2的体内定位仅限于血管化良好区域的细胞群。因此，B6.2和B72.3的体内积累虽然在数量上相似，但在LS 174 T肿瘤内显示出非常不同的空间分布。在克劳泽肿瘤中B6.2的体内结合仅限于相当均匀地分散在整个肿瘤中的小细胞簇。没有证据表明，在体外培养的肿瘤切片与B6.2表明，区域摄取的异质性可能是由于抗体输送的差异，这种富含抗原的病灶的存在。这种类型的信息可以提供一个合理的基础上选择适当的治疗同位素的放射免疫治疗研究使用这些和其他肿瘤模型。
SummaryThe targeting of monoclonal antibodies to human tumor xenografts in nude mice was investigated by analysis of the cellular distribution of two radioiodinated monoclonal antibodies, B6.2 and B72.3, which recognize different tumor-associated antigens. The time course of distribution of each antibody within Clouser human mammary carcinoma (B6.2 positive, B72.3 negative) and LS174T human colorectal carcinoma (B6.2 positive, B72.3 positive) following i. v. injections was compared using autoradiographic techniques, which were also used to determine the pattern of binding after in vitro incubation with radiolabeled antibody. Both in vivo and in vitro localization of 125I-B72.3 in LS174T were characterized by the binding of antibody to antigen-rich mucin globules. In contrast, in vivo localization of B6.2 was restricted to groups of cells in well vascularized regions. Thus, the in vivo accumulations of B6.2 and B72.3 although quantitatively similar showed very different spatial distributions within LS174T tumors. The in vivo binding of B6.2 in Clouser tumors was restricted to small clusters of cells scattered fairly evenly throughout the tumor. There was no evidence for the presence of such antigen-rich foci after in vitro incubation of tumor sections with B6.2 suggesting that heterogeneity of regional uptake may be due to differences in antibody delivery. This type of information may provide a rational basis for the selection of appropriate therapeutic isotopes for radioimmunotherapy studies using these and other tumor models.
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使用 L-131 标记的单克隆抗体对黑色素瘤进行成像。
DOI：
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发表时间：
1983
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Larson,SM;Brown,JP;Wright,PW;Carrasquillo,JA;Hellstrom,I;Hellstrom,KE
通讯作者：
Hellstrom,KE
通过单克隆抗体对人类肿瘤异种移植物进行放射免疫检测。
影响因子：
11.2
作者：
Herlyn,D;Powe,J;Alavi,A;Mattis,JA;Herlyn,M;Ernst,C;Vaum,R;Koprowski,H
通讯作者：
Koprowski,H
通过高效液相色谱法纯化和分析单克隆抗体。
影响因子：
--
作者：
Burchiel,SW
通讯作者：
Burchiel,SW
影响因子：
15.9
作者：
LARSON, SM;CARRASQUILLO, JA;HELLSTROM, I
通讯作者：
HELLSTROM, I