Induction of necrotizing funisitis by fetal administration of intravenous granulocyte-colony stimulating factor and intra-amniotic endotoxin in premature fetal sheep
Induction of necrotizing funisitis by fetal administration of intravenous granulocyte-colony stimulating factor and intra-amniotic endotoxin in premature fetal sheep
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DOI：
10.1203/pdr.0b013e31815991bf
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发表时间：
2007-12-01
影响因子：
3.6
通讯作者：
Kobayashi, Yoshiyasu
中科院分区：
文献类型：
--
作者：
Watanabe, Tatsuya;Matsuda, Tadashi;Kobayashi, Yoshiyasu
本研究的目的是确定实验性子宫内炎症是否能诱导坏死性脐带炎，一种严重的脐带慢性炎症。将胎儿随机分为4组，每组4只，分别于孕125-129天静脉滴注粒细胞集落刺激因子（G-CSF）50 μ g/d（G-CSF组），孕127天羊膜腔内注入内毒素20 mg（内毒素组），G-CSF+内毒素组（G-CSF +内毒素组），对照组（仅注入生理盐水组）。在妊娠第130天，对脐带进行组织学分析，对炎症程度进行评分，并进行统计学比较。出生时，G-CSF组和G-CSF +内毒素组的外周血多形核白细胞计数显著高于内毒素组和对照组（p < 0.05）。G-CSF +内毒素组脐带炎症评分明显高于其他3组（p < 0.05）。G-CSF +内毒素组所有胎仔均出现坏死性精索炎，而其他三组胎仔均未出现坏死性精索炎。在脐带中激活前，血液中多形核白细胞的增加可能是实验诱导坏死性脐带炎所必需的。
The purpose of the present study was to determine whether experimental intrauterine inflammation could induce necrotizing funisitis, a severe, chronic inflammation of the umbilical cord. Fetuses, randomly divided into four groups (n = 4 each), were infused with 50 mu g/d of granulocyte-colony stimulating factor (G-CSF) intravenously on d 125-129 of gestation (G-CSF group), 20 mg of endotoxin into the amniotic cavity on d 127 gestation (endotoxin group), both G-CSF and endotoxin (G-CSF + endotoxin group), or only saline (control group). On d 130 of gestation, the umbilical cords were processed for histologic analysis, scored for degree of inflammation, and compared statistically. At birth, the blood polymorphonuclear leukocyte counts in G-CSF and G-CSF + endotoxin groups were significantly higher than those in endotoxin and control groups (p < 0.05). The inflammatory score of the umbilical cord in G-CSF + endotoxin group was significantly higher than those in the other three groups (p < 0.05). All the fetuses in G-CSF + endotoxin group had necrotizing funisitis, but none of the fetuses in the other three groups developed this condition. An increase in blood polymorphonuclear leukocytes before their activation in the umbilical cord is probably essential for experimentally inducing necrotizing funisitis.