Wnt10b promotes growth of hair follicles via a canonical Wnt signalling pathway
Wnt10b promotes growth of hair follicles via a canonical Wnt signalling pathway
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DOI：
10.1111/j.1365-2230.2011.04019.x
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发表时间：
2011-07-01
影响因子：
4.1
通讯作者：
Yang, T.
中科院分区：
文献类型：
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作者：
Li, Y. -H.;Zhang, K.;Yang, T.
背景。Wnt10b（无翅相关小鼠乳腺肿瘤病毒整合位点10b）在包括毛囊发育在内的广泛生物活动中发挥多种作用。探讨Wnt10b在出生后毛囊生长中的作用。腺病毒载体AdWnt10b。AdGFP、AdGFP + AdREP、AdWnt10b + AdErzB、AdWnt10b + AdSimBC分别与肉芽菌共培养。免疫组化法检测Wnt10b、β -catenin、Left1原位蛋白表达，5-溴-2-脱氧尿苷（BrdU）标记法检测毛囊增殖情况。利用pcr微阵列技术检测了Wnt信号分子在毛囊生长的三个阶段的存在。adwnt10b感染的细胞能够分泌具有生物活性的Wnt10b，当将其添加到基础培养基中时，疣体长得比在对照培养基或含有典型Wnt信号拮抗剂的培养基中快。Wnt10b的原位蛋白表达与β -catenin和Lef1一致。Wnt10b的表达位点与BrdU标记的增殖细胞在毛囊中的表达位点基本一致。Wnt10b可能通过典型的Wnt信号通路诱导毛囊从休止期到生长期的转换，从而促进基质细胞的增殖，从而促进毛囊生长。
Background. Wnt10b (wingless-related mouse mammary tumour virus integration site 10b) plays various roles in a wide range of biological actions, including hair-follicle development.Aim. To assess the roles that Wnt10b plays in postnatal hair-follicle growth.Methods. Adenovirus vectors AdWnt10b. AdGFP, AdGFP plus AdREP, AdWnt10b plus AdErzB, and AdWnt10b plus AdSimBC were co-cultured separately with vibrissae. In situ protein expression of Wnt10b, beta-catenin and Left1 was determined by immunohistochemistry, and the proliferation status of the hair follicle was detected by 5-bromo-2-deoxyuridine (BrdU) labelling. The presence of Wnt signalling molecules in the three stages of hair-follicle growth was detected by PCR-based microarray.Results. AdWnt10b-infected cells were able to secrete bioactive Wnt10b, and when this was added into the basal medium, the vibrissae grew faster than in control medium or in medium containing canonical Wnt signalling antagonists. The in situ protein expression of Wnt10b was consistent with that of beta-catenin and Lef1. The expression locus of Wnt10b was almost the same as the proliferating cells labelled by BrdU in the anagen hair follicle.Conclusions. Wnt10b may promote hair-follicle growth by inducing the switch from telogen to anagen via a canonical Wnt signalling pathway to promote the proliferation of matrix cells.