Phosphoglycerate kinase 1 as a promoter of metastasis in colon cancer
Phosphoglycerate kinase 1 as a promoter of metastasis in colon cancer
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DOI：
10.3892/ijo.2013.1971
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发表时间：
2013-08-01
影响因子：
5.2
通讯作者：
Zieker, Derek
中科院分区：
文献类型：
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作者：
Ahmad, Sufian S.;Glatzle, Joerg;Zieker, Derek
由于实体癌中肿瘤内缺氧引起的氧化应激已被证明与死亡率增加相关。磷酸甘油酸激酶1（PGK 1）是糖酵解途径的一种酶，其由缺氧诱导因子-1 α（HIF-1 α）调节，并且已经描述了其在几种恶性肿瘤的肿瘤进展和转移中的作用。我们研究了转移性和非转移性结肠癌之间PGK 1的表达是否不同。我们通过聚合酶链反应（PCR）和免疫组化比较了有或无转移的结肠癌患者PGK 1的表达。使用来自HCT 116细胞系的分离株进行微阵列分析以测试PGK 1沉默后改变的基因表达。PCR结果显示转移患者结肠癌组织中PGK 1的表达较无转移患者增加（倍数变化2.6，p
Oxidative stress due to intratumoral hypoxia in solid cancer has been shown to be associated with increased mortality. Phosphoglycerate kinase 1 (PGK1) is an enzyme of the glycolytic pathway, which is regulated by hypoxia-inducible factor-1 alpha (HIF-1 alpha) and has been described for its role in tumor progression and metastasis in several malignancies. We investigated whether the expression of PGK1 varies between metastatic and non-metastatic colon cancer. We compared PGK1 expression in colon cancer patients either with or without metastasis via polymerase chain reaction (PCR) and immunohistochemistry. Microarray analysis was performed to test altered gene expression after PGK1 silencing, using isolates from HCT116 cell lines. PCR results showed an increased expression of PGK1 in colon cancer tissue from metastatic patients in comparison to patients with no metastasis (fold change 2.6, p