Derivation of chicken primordial germ cells using an indirect Co-culture system
Derivation of chicken primordial germ cells using an indirect Co-culture system
复制标题
使用间接共培养系统衍生鸡原始生殖细胞
DOI：
10.1016/j.theriogenology.2018.09.017
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发表时间：
2019-01-01
期刊：
影响因子：
2.8
通讯作者：
Lu, Yangqing
中科院分区：
文献类型：
--
作者：
Xie, Long;Lu, Zhenping;Lu, Yangqing
原始生殖细胞(PGC)是包括转基因动物在内的禽类研究的重要遗传资源。然而，经过长期培养后，鸡PGCs增殖缓慢，并逐渐丧失种系能力，这阻碍了其在禽类生物技术中的应用。因此，我们开发了一种可靠的方法，利用间接共培养系统分离和快速繁殖PGCs。从一对鸡胚性腺中提取的PGC5在2周内扩增至1×10(6)，且未观察到性别偏见。这些生殖细胞具有很高的生殖系传播能力，并在注射到鸡胚胎后产生供体来源的后代。该系统允许高效的鸡种基因库，并可以通过基因组编辑促进具有所需表型的鸡的生产。(C)2018 Elsevier Inc.保留所有权利。
Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after longterm culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGC5 derived from a pair of embryonic chicken gonads were expanded to 1 x 10(6) within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing. (C) 2018 Elsevier Inc. All rights reserved.