Phosphorescent Light-Emitting Iridium Complexes Serve as a Hypoxia-Sensing Probe for Tumor Imaging in Living Animals
Phosphorescent Light-Emitting Iridium Complexes Serve as a Hypoxia-Sensing Probe for Tumor Imaging in Living Animals
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DOI：
10.1158/0008-5472.can-09-3948
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发表时间：
2010-06-01
期刊：
影响因子：
11.2
通讯作者：
Takeuchi, Toshiyuki
中科院分区：
文献类型：
--
作者：
Zhang, Shaojuan;Hosaka, Masahiro;Takeuchi, Toshiyuki
Ir络合物是一种很有前途的有机发光二极管材料，可用于下一代视频显示器，发出被氧猝灭的磷光。我们利用这种氧猝灭特性对肿瘤缺氧进行成像。红光发射Ir(Btp)(2)(Acac)(Btp)(Acac)(Btp)(BTP)在培养的细胞系中表现出低氧依赖的发光，其强度与低氧诱导因子1α图像平行进一步将BTP应用于5只荷瘤裸鼠的成像。所有肿瘤在注射后5分钟都呈现出明亮的BTP发射图像。最小图像识别大小与直径接近2 mm。通过形态观察和磷光寿命测量，BTP似乎定位于肿瘤细胞。由于BTP易于修饰，我们合成了具有较长激发/发射波长的BTP类似物。其中之一，BTPHSA，描绘了移植到距离皮肤表面6到7毫米深的肿瘤的清晰成像。我们认为，Ir络合物材料在肿瘤组织等缺氧性损伤成像方面具有巨大的潜力。癌症资源；70(11)；4490-8。(C)2010年AACR。
Iridium complex is a promising organic light-emitting diode material for next generation video displays that emits phosphorescence quenched by oxygen. We used this oxygen-quenching feature for imaging tumor hypoxia. Red light-emitting Ir(btp)(2)(acac) (BTP) presented hypoxia-dependent light emission in culture cell lines, whose intensity was in parallel with hypoxia-inducible factor-1 alpha images. BTP was further applied to imaging five nude mouse transplanted with tumors. All tumors presented a bright BTP-emitting image even 5 minutes after injection. The minimal image recognition size was similar to 2 mm in diameter. By morphologic examination and phosphorescence lifetime measurement, BTP appeared to localize to the tumor cells. Because BTP is easily modifiable, we synthesized BTP analogues with a longer excitation/emission wavelength. One of them, BTPHSA, depicted clear imaging from tumors transplanted 6 to 7 mm deep from the skin surface. We suggest that iridium complex materials have a vast potential for imaging hypoxic lesions such as tumor tissues. Cancer Res; 70(11); 4490-8. (c) 2010 AACR.