SAGE identification and fluorescence imaging analysis of genes and transcripts in melanomas and precursor lesions
SAGE identification and fluorescence imaging analysis of genes and transcripts in melanomas and precursor lesions
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DOI：
10.4161/cbt.3.1.661
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发表时间：
2004-01-01
影响因子：
3.6
通讯作者：
Becker, D
中科院分区：
文献类型：
--
作者：
Smith, AP;Weeraratna, AT;Becker, D
散发性和家族性黑色素瘤在大约40-80%的病例中以逐步的方式发展；然而，控制从粒细胞前体病变到早期和晚期黑色素瘤进展的遗传事件仍然很大程度上是未知的。在目前的研究中，我们提供了对最近产生的四个原发和转移性黑色素瘤基因表达序列分析(SAGE)文库中识别的基因的分析。除了与功能未知的转录本或未鉴定的转录本相对应的SAGE标签外，还发现了迄今尚未被证明在早期和晚期黑色素瘤和/或黑色素瘤前体病变中表达或具有功能的已知基因。用菁染料标记的抗体和寡核苷酸进行荧光成像分析，我们建立了ATM、HEL10、PKD1、KAl11、IL-10R和假想蛋白Fla 1151在痣和黑色素瘤标本中的表达模式。
Melanomas of sporadic and familial origin develop in a stepwise fashion in approximately 40-80% of all cases; yet, the genetic events governing the progression from nevocytic precursor lesions to early and advanced-stage melanomas remain largely unknown. In the present study, we provide an analysis of genes that were identified in four recently generated primary and metastatic melanoma Serial Analysis of Gene Expression (SAGE) libraries. In addition to SAGE tags corresponding to transcripts with unknown function, or to unidentified transcripts, known genes were identified that hitherto have not been shown to be expressed or have a function in early and advanced-stage melanomas and/or melanoma precursor lesions. Conducting fluorescence imaging analysis with cyanine dye-conjugated antibodies and oligonucleotides, we established the expression pattern of ATM, HEl10, PKD1, KAl11, IL-10R, and hypothetical protein FLA 1151 in nevus and melanoma specimens.