Characterization of Borrelia burgdorferi isolates by restriction endonuclease analysis and DNA hybridization.
Characterization of Borrelia burgdorferi isolates by restriction endonuclease analysis and DNA hybridization.
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通过限制性内切核酸酶分析和 DNA 杂交鉴定伯氏疏螺旋体分离株。
DOI：
10.1128/jcm.27.4.636-639.1989
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发表时间：
1989
影响因子：
9.4
通讯作者：
Johnson,RC
中科院分区：
文献类型：
--
作者：
LeFebvre,RB;Perng,GC;Johnson,RC
用限制性内切酶分析和标记B的DNA杂交对几个来自北美和欧洲的伯氏疏螺旋体分离株的基因组进行了表征。burgdorferi全细胞DNA（菌株ATCC 35210）。在这些分离株中观察到几种不同的限制和同源模式，表明该属和种内的基因型异质性。结果表明，B. Burgdorferi全细胞DNA可能是鉴定莱姆病病原体菌株或基因型的可靠和准确的方法。
Genomes of several Borrelia burgdorferi isolates from North America and Europe were characterized by restriction endonuclease analysis and DNA hybridization using labeled B. burgdorferi whole-cell DNA (strain ATCC 35210). Several different restriction and homology patterns were observed among these isolates, indicating genotypic heterogeneity within this genus and species. It was concluded from this study that restriction endonuclease analysis of B. burgdorferi whole-cell DNA may be a reliable and accurate method for identifying strains or genotypes of the Lyme disease agent.