Aerosol-jet-printed graphene electrochemical histamine sensors for food safety monitoring
Aerosol-jet-printed graphene electrochemical histamine sensors for food safety monitoring
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DOI：
10.1088/2053-1583/ab8919
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发表时间：
2020-07-01
期刊：
影响因子：
5.5
通讯作者：
Claussen, Jonathan C.
中科院分区：
文献类型：
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作者：
Parate, Kshama;Pola, Cicero C.;Claussen, Jonathan C.
石墨烯等碳纳米材料表现出独特的材料特性，包括高电导率、表面积和生物相容性，有可能显着提高电化学传感器的性能。由于现场电化学传感器通常是一次性的，因此它们需要适合低成本、高通量和可扩展制造的材料。基于低产率化学气相沉积技术的常规石墨烯装置对于此类应用来说太昂贵，而低成本替代方案（例如丝网印刷和喷墨印刷）不具有对电极几何形状的足够控制以实现有利的电化学传感器性能。在这项工作中，气溶胶喷射印刷（AJP）是用来创建高分辨率（类似于40 μ m线宽）的叉指电极（IDE）的柔性基板上，然后转换成组胺传感器通过共价连接单克隆抗体的氧部分通过CO（2）热退火过程中产生的石墨烯表面。所得的电化学传感器表现出广泛的组胺感测范围为6.25-200 ppm（56.25 μ M-1.8 mM）和低检测限为3.41 ppm（30.7 μ M）内的实际金枪鱼肉汤样品。这些传感器度量是重要的，因为鱼中组胺水平超过50 ppm会引起不良健康影响，包括严重的过敏反应（例如，鲭鱼食物中毒）。除了这里介绍的组胺案例研究之外，AJP和功能化过程可能会推广到各种各样的传感应用，包括环境毒素检测，食源性病原体检测，可穿戴健康监测和健康诊断。
Carbon nanomaterials such as graphene exhibit unique material properties including high electrical conductivity, surface area, and biocompatibility that have the potential to significantly improve the performance of electrochemical sensors. Since in-field electrochemical sensors are typically disposable, they require materials that are amenable to low-cost, high-throughput, and scalable manufacturing. Conventional graphene devices based on low-yield chemical vapor deposition techniques are too expensive for such applications, while low-cost alternatives such as screen and inkjet printing do not possess sufficient control over electrode geometry to achieve favorable electrochemical sensor performance. In this work, aerosol jet printing (AJP) is used to create high-resolution (similar to 40 mu m line width) interdigitated electrodes (IDEs) on flexible substrates, which are then converted into histamine sensors by covalently linking monoclonal antibodies to oxygen moieties created on the graphene surface through a CO(2)thermal annealing process. The resulting electrochemical sensors exhibit a wide histamine sensing range of 6.25-200 ppm (56.25 mu M-1.8 mM) and a low detection limit of 3.41 ppm (30.7 mu M) within actual tuna broth samples. These sensor metrics are significant since histamine levels over 50 ppm in fish induce adverse health effects including severe allergic reactions (e.g. Scombroid food poisoning). Beyond the histamine case study presented here, the AJP and functionalization process can likely be generalized to a diverse range of sensing applications including environmental toxin detection, foodborne pathogen detection, wearable health monitoring, and health diagnostics.