Disrupting the wall accumulation of human sperm cells by artificial corrugation.
Disrupting the wall accumulation of human sperm cells by artificial corrugation.
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DOI：
10.1063/1.4918979
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发表时间：
2015-01
期刊：
影响因子：
3.2
通讯作者：
H. Guidobaldi;Y. Jeyaram;C. Condat;M. Oviedo;I. Berdakin;V. Moshchalkov;L. Giojalas;A. Silhanek;V. Marconi
中科院分区：
文献类型：
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作者：
H. Guidobaldi;Y. Jeyaram;C. Condat;M. Oviedo;I. Berdakin;V. Moshchalkov;L. Giojalas;A. Silhanek;V. Marconi
许多自行式微生物会被吸引到表面。这使得它们在受限几何中的动力学与在整体中观察到的非常不同。沿壁游动有利于引导和分选细胞，但如果需要均匀的群体，例如计数微室，则可能是有害的。在这项工作中，我们描述了人类精子细胞的运动特征，∼60μm长，强烈限制在∼25μm的浅室。我们研究了限制表面之间的细胞轨迹的性质以及它们在边界附近的积累。观察到的细胞轨迹由一系列准圆形和准线性段组成。这表明细胞沿着顶部和底部表面附近的间歇性陷阱路径，两个表面之间的准自由运动延伸隔开，深度分辨共聚焦显微镜研究证实了这一点。我们发现，在横向边界引入人工花瓣形状的波纹消除了细胞在边界附近聚集的趋势，我们假设这一效果可能对微流体在生物医学中的应用有价值。
Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very different from that observed in the bulk. Swimming along walls is beneficial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells ∼60 μm long, strongly confined to ∼25 μm shallow chambers. We investigate the nature of the cell trajectories between the confining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confirmed by depth resolved confocal microscopy studies. We show that the introduction of artificial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an effect which we hypothesize may be valuable for microfluidic applications in biomedicine.