A Near-Complete Spatial Map of Olfactory Receptors in the Mouse Main Olfactory Epithelium.
A Near-Complete Spatial Map of Olfactory Receptors in the Mouse Main Olfactory Epithelium.
复制标题
小鼠主嗅上皮中嗅觉受体的近乎完整的空间图。
DOI：
10.1093/chemse/bjy030
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Xie,XiaoliangSunney
中科院分区：
文献类型：
--
作者：
Tan,Longzhi;Xie,XiaoliangSunney
哺乳动物鼻子的不同区域闻到不同的气味。在小鼠嗅觉系统中，>；1000嗅觉受体(ORs)沿背内侧-腹外侧(DV)轴的空间调控表达形成了主嗅觉上皮(MOE)的拓扑图。然而，大多数OR沿DV轴的位置目前尚不清楚。通过对12个分离的MOE片段的mRNA测序，我们绘制出了1033个OR基因中的DV位置--用1-5的“区带指数”来量化，估计误差为0.3区带指数。我们的图谱覆盖了81%的完整OR基因和99.4%的OR mRNA丰度。空间调控倾向于沿着染色体逐渐变化。我们进一步鉴定了可能沿DV轴表现出空间表达的非OR基因。
Different regions of the mammalian nose smell different odors. In the mouse olfactory system, spatially regulated expression of >1000 olfactory receptors (ORs) along the dorsomedial–ventrolateral (DV) axis forms a topological map in the main olfactory epithelium (MOE). However, the locations of most ORs along the DV axis are currently unknown. By sequencing mRNA of 12 isolated MOE pieces, we mapped out the DV locations—as quantified by “zone indices” on a scale of 1–5—of 1033 OR genes with an estimated error of 0.3 zone indices. Our map covered 81% of all intact OR genes and 99.4% of the total OR mRNA abundance. Spatial regulation tended to vary gradually along chromosomes. We further identified putative non-OR genes that may exhibit spatial expression along the DV axis.