RNA Sequencing Identifies a Common Physiology in Vancomycin- and Ciprofloxacin-Tolerant Staphylococcus aureus Induced by ileS Mutations
RNA Sequencing Identifies a Common Physiology in Vancomycin- and Ciprofloxacin-Tolerant Staphylococcus aureus Induced by ileS Mutations
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RNA 测序鉴定了 ileS 突变诱导的耐万古霉素和环丙沙星金黄色葡萄球菌的常见生理学
DOI：
10.1128/aac.00827-20
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发表时间：
2020
影响因子：
4.9
通讯作者：
Hiramatsu K
中科院分区：
文献类型：
--
作者：
Singh M;Matsuo M;Sasaki T;Hishinuma T;Yamamoto N;Morimoto Y;Kirikae T;Hiramatsu K
关于突变在金黄色葡萄球菌中诱导万古霉素耐受的机制知之甚少。这项研究表明万古霉素耐受突变体和 IleRS 抑制剂处理的亲本的转录组谱相似。值得注意的是，诱导严格反应的ileSandrelA 被上调。相同的机制导致万古霉素和环丙沙星的交叉耐受。这些发现表明，金黄色葡萄球菌中ileS突变后不带电的异亮氨酰-tRNA的积累是造成药物耐受性的原因。
Little is known about the mechanisms by whichileSmutations induce vancomycin tolerance in Staphylococcus aureus. This study showed that transcriptome profiles were similar in vancomycin-tolerant mutants and the IleRS-inhibitor-treated parent. Notably,ileSandrelA, which induce a stringent response, were upregulated. The same mechanism was responsible for cross-tolerance to vancomycin and ciprofloxacin. These findings suggest that the accumulation of uncharged isoleucyl-tRNA followingileSmutations in S. aureus was responsible for drug tolerance.