Extracellular serotonin levels in the medullary reticular formation during normal sleep and after REM sleep deprivation

Extracellular serotonin levels in the medullary reticular formation during normal sleep and after REM sleep deprivation
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正常睡眠期间和快速眼动睡眠剥夺后髓质网状结构中的细胞外血清素水平

DOI：
10.1016/s0006-8993(01)03209-7
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发表时间：
2001
期刊：
Brain Research
影响因子：
2.9
通讯作者：
R. Salín
R. Salín
中科院分区：
医学3区
文献类型：
--
作者：
C. Blanco;R. Salín

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据推测，快速眼动 (REM) 睡眠是由 REM 睡眠生成神经元和 REM 睡眠抑制神经元的活动引起的。中缝背核 (DRN) 的血清素能 (5-HT) 神经元代表了一种快速眼动睡眠抑制神经元，因为它们在快速眼动睡眠期间保持沉默。与 5-HT 神经元活动的减少一致，与清醒时相比，快速眼动睡眠期间大脑细胞外 5-HT 水平较低。目前尚不清楚血清素释放是否也会因快速眼动睡眠反弹而减少。使用微透析采样与 HPLC-ECD 相结合，我们测量了自由行为大鼠在正常睡眠、快速眼动睡眠剥夺以及快速眼动睡眠反弹期间内侧髓质网状结构 (mMRF) 中 5-HT 及其代谢物 (5-HIAA) 的细胞外水平。我们发现 REM 睡眠剥夺会显着降低 5-HT 和 5-HIAA 水平。在快速眼动睡眠反弹期间，5-HT 释放量保持减少，但其主要代谢物的细胞外水平增加。此外，即使在快速眼动睡眠反弹期间，睡眠期间的 5-HT 释放量也比清醒时低。总而言之，这些数据支持 5-HT 神经传递对 REM 睡眠表达的许可作用。
Rapid eye movement (REM) sleep is hypothesized to result from the activity of REM sleep-generating and REM sleep-inhibiting neurons. The serotoninergic (5-HT) neurons of the dorsal raphe nucleus (DRN) represents one such population of REM-sleep inhibiting neurons since they are silent during REM sleep. Consistent with the decrease in activity of 5-HT neurons, the brain extracellular levels of 5-HT are lower during REM sleep compared to wakefulness. It is not known whether serotonin release is also reduced as a consequence of REM sleep rebound. Using microdialysis sampling coupled to HPLC–ECD, we measured the extracellular levels of 5-HT and its metabolite (5-HIAA) in the medial medullary reticular formation (mMRF) of freely behaving rats during normal sleep, REM sleep deprivation as well as during REM sleep rebound. We found that the levels 5-HT and 5-HIAA were significantly decreased by REM sleep deprivation. The reduction of 5-HT release was maintained during REM sleep rebound but the extracellular level of its main metabolite was increased. In addition, even during REM sleep rebound, 5-HT release during sleep was low compared to wakefulness. Taken together these data support the permissive role of 5-HT neurotransmission for REM sleep expression.
Behavioral state-related changes of extracellular serotonin concentration in the pedunculopontine tegmental nucleus: a microdialysis study in freely moving animals.
脚桥被盖核中细胞外血清素浓度的行为状态相关变化：自由活动动物的微透析研究。
DOI： --
发表时间： 1999
期刊： Sleep research online [electronic resource] : SRO.
影响因子： --
作者：
Strecker,RE;Thakkar,MM;Porkka-Heiskanen,T;Dauphin,LJ;Bjorkum,AA;McCarley,RW
通讯作者： McCarley,RW