Binding of Candida albicans yeast cells to mouse popliteal lymph node tissue is mediated by macrophages.
Binding of Candida albicans yeast cells to mouse popliteal lymph node tissue is mediated by macrophages.
复制标题
白色念珠菌酵母细胞与小鼠腘淋巴结组织的结合是由巨噬细胞介导的。
DOI：
10.1128/iai.61.8.3244-3249.1993
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发表时间：
1993
影响因子：
3.1
通讯作者：
Cutler,JE
中科院分区：
文献类型：
--
作者：
Han,Y;vanRooijen,N;Cutler,JE
我们以前报道过白色念珠菌酵母细胞粘附在小鼠淋巴结组织中富含巨噬细胞的髓质和被膜下窦区。为了确定酵母细胞-淋巴结相互作用是否由巨噬细胞介导，研究了巨噬细胞的特异性消除对酵母细胞结合的影响，并将酵母细胞粘附与淋巴结细胞摄取印度墨水相关。使用含有脂质体的二氯亚甲基二磷酸盐（L-Cl 2 MDP）消除巨噬细胞。在小鼠的后足垫中注射L-Cl 2 MDP制剂，5天后取出腘淋巴结，并通过离体结合试验测定酵母细胞粘附。作为对照，使用来自接受单独的磷酸盐缓冲盐水（PBS）或含有脂质体的PBS的足垫注射的小鼠的淋巴结。在组织免疫染色中使用巨噬细胞和嗜中性粒细胞特异性单克隆抗体表明，L-Cl 2 MDP治疗消除了腘淋巴结髓质和被膜下窦区域的巨噬细胞，但不消除中性粒细胞。与对照动物的淋巴结相比，L-Cl 2 MDP处理小鼠的淋巴结发生酵母细胞粘附的显著减少。L-Cl 2 MDP处理的小鼠和对照小鼠的淋巴结-酵母细胞结合模式是相同的，与小鼠品系、性别或T细胞能力无关。印度墨水实验的结果显示，酵母粘附和细胞的印度墨水染色之间存在很强的相关性，在实验中，印度墨水被注射到小鼠的足垫中，并迅速被腘淋巴结巨噬细胞吸收。此外，酵母细胞与正常小鼠淋巴结组织的相互作用没有受到两种细胞外基质蛋白，纤连蛋白和层粘连蛋白，在离体粘附试验中的加入显着影响。这些数据表明，髓质和被膜下窦淋巴结巨噬细胞表达的粘附系统类似于小鼠脾边缘区巨噬细胞的描述。
We previously reported that Candida albicans yeast cells adhere to the macrophage-rich medullary and subcapsular sinus areas of mouse lymph node tissue. To determine whether the yeast cell-lymph node interaction is mediated by macrophages, the effect of specific elimination of macrophages on yeast cell binding was studied, and yeast cell adherence was correlated with the ingestion of India ink by lymph node cells. Macrophage elimination was done by use of liposome-containing dichloromethylene diphosphonate (L-Cl2MDP). Mice were injected in the hind footpads with the L-Cl2MDP preparation, popliteal lymph nodes were removed 5 days later, and yeast cell adherence was determined by an ex vivo binding assay. As controls, lymph nodes from mice that received footpad injections of either phosphate-buffered saline (PBS) alone or liposome-containing PBS were used. Use of macrophage- and neutrophil-specific monoclonal antibodies in tissue immunostaining showed that the L-Cl2MDP treatment eliminated macrophages but not neutrophils from the medullary and subcapsular sinus areas of the popliteal lymph nodes. A striking reduction of yeast cell adherence occurred with lymph nodes from L-Cl2MDP-treated mice compared with lymph nodes from control animals. The lymph node-yeast cell binding patterns of L-Cl2MDP-treated and control mice were the same regardless of mouse strain, sex, or T-cell competency. Results of India ink experiments, in which India ink was injected into footpads of mice and was rapidly taken up by popliteal lymph node macrophages, showed a strong correlation between yeast adherence and India ink staining of cells. In addition, the interaction of yeast cells with lymph node tissue from normal mice was not significantly affected by the addition of two extracellular matrix proteins, fibronectin and laminin, during the ex vivo adherence assay. These data indicate that medullary and subcapsular sinus lymph node macrophages express an adhesion system similar to that described for mouse splenic marginal zone macrophages.