Roles of the C.elegans cyclophilin-like protein MOG-6 in MEP-1 binding and germline fates
Roles of the C.elegans cyclophilin-like protein MOG-6 in MEP-1 binding and germline fates
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DOI：
10.1242/dev.01154
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发表时间：
2004-06-01
期刊：
影响因子：
4.6
通讯作者：
Puoti, A
中科院分区：
文献类型：
--
作者：
Belfiore, M;Pugnale, P;Puoti, A
秀丽隐杆线虫雌雄同体从精子发生到卵子发生的转变需要 mog-6，它在转录后抑制 fem-3 RNA。在这项研究中，我们证明 mog-6 编码不同的核亲环蛋白，因为它在精子-卵母细胞转换中的功能不需要保守结构域。 MOG-6 通过两个独立的结构域与核锌指蛋白 MEP-1 结合，这两个结构域对于 MOG-6 在精子-卵母细胞转换中的作用至关重要。我们认为 MOG-6 具有与普遍的亲环蛋白不同的功能，并且它与 MEP-1 的结合对于种系性别决定至关重要。最后，我们发现gld-3 mog-6突变体会产生种系肿瘤，这表明mog-6可能在有丝分裂和减数分裂之间的决定中发挥作用。
The switch from spermatogenesis to oogenesis in the Caenorhabditis elegans hermaphrodite requires mog-6, which post-transcriptionally represses the fem-3 RNA. In this study, we show that mog-6 codes for a divergent nuclear cyclophilin, in that a conserved domain is not required for its function in the sperm-oocyte switch. MOG-6 binds to the nuclear zinc finger protein MEP-1 through two separate domains that are essential for the role of MOG-6 in the sperm-oocyte switch. We propose that MOG-6 has a function distinct from that of prevailing cyclophilins and that its binding to MEP-1 is essential for germline sex determination. Finally, we found that gld-3 mog-6 mutants develop germline tumors, suggesting that mog-6 might function in the decision between mitosis and meiosis.