Carbon nanosphere-based fluorescence aptasensor for targeted detection of breast cancer cell MCF-7
Carbon nanosphere-based fluorescence aptasensor for targeted detection of breast cancer cell MCF-7
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基于碳纳米球的荧光适体传感器靶向检测乳腺癌细胞MCF-7
DOI：
10.1016/j.talanta.2018.03.045
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
Wang Zhifei
中科院分区：
文献类型：
--
作者：
Yang D;an;Liu Mei;Xu Jing;Yang Chao;Wang Xiaoxiao;Lou Yongbing;He Nongyue;Wang Zhifei
在这项工作中，开发了基于碳纳米球（CNS）的荧光“关闭/打开”适配器，用于通过与FAM（染料）标记的粘蛋白1（MUC 1）适配体P0（P0-FAM）缀合来靶向检测乳腺癌细胞MCF-7，该适配体可以识别在MCF-7表面上过表达的MUC 1蛋白。与其他碳基荧光猝灭材料不同的是，葡萄糖碳化法制备的碳纳米管不仅具有较高的荧光猝灭效率（98.8%），而且细胞毒性可忽略不计（在0-1 mg/mL浓度范围内，是传统碳纳米管或氧化石墨烯（0-100 µg/mL）的10倍）。对于肿瘤抗原MUC 1模拟物的检测，所得荧光强度在0-6 μM范围内几乎呈线性增加，检测限（LOD）为25 nM。
In this work, carbon nanosphere (CNS)-based fluorescence “turn off/on” aptasensor was developed for targeted detection of breast cancer cell MCF-7 by conjugation with FAM (a dye)-labeled mucin1 (MUC1) aptamer P0 (P0-FAM), which can recognize MUC1 protein overexpressed on the surface of MCF-7. Different from other carbon based fluorescence quenching materials, CNSs prepared by the carbonization of glucose not only have the high fluorescence quenching efficiency (98.8%), but also possess negligible cytotoxicity (in the concentration range of 0–1 mg/mL, which is 10 times higher than that of traditional carbon nanotubes or graphene oxide (0–100 µg/mL)). As for the detection of the mimic of the tumor antigen MUC1, the resulting fluorescence intensity increases nearly linearly in the range of 0–6 μM with the limit of detection (LOD) of 25 nM.