Effects of Sympathetic Innervation Loss on Mandibular Distraction Osteogenesis
Effects of Sympathetic Innervation Loss on Mandibular Distraction Osteogenesis
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DOI：
10.1097/scs.0b013e31825daab2
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发表时间：
2012-09
影响因子：
0.9
通讯作者：
Tao Wang;Jian-gang Cao;Zhao-jie Du;Ya-bo Zhang;Yanpu Liu;Lei Wang;De-lin Lei
中科院分区：
文献类型：
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作者：
Tao Wang;Jian-gang Cao;Zhao-jie Du;Ya-bo Zhang;Yanpu Liu;Lei Wang;De-lin Lei
摘要交感神经系统对骨量有重要的调节作用。然而，交感神经系统在牵张成骨（DO）中的作用尚不清楚。在这里，我们表明，交感神经系统在下颌骨DO中起着重要的作用。将30只雄性SD大鼠随机分为2组。对照组（A组）15只，行右侧下颌骨牵开术。实验组（B组）15只，行双侧颈交感神经干切断和右侧下颌骨DO。术后1、14、28 d分别对牵引间隙新生骨痂进行大体定量观察、显微CT骨形态学分析和苏木精-伊红染色。采用SPSS12.0软件包进行统计学分析。在第1天和第14天，大体观察显示，实验组比对照组有更多的连续骨形成。实验组骨密度、骨体积/总体积比、骨小梁数及组织学检查均显著高于对照组（P < 0.05）。两组间的巩固时间无显著差异，均为28天。提示交感神经支配的缺失可促进下颌骨DO和新骨形成，交感神经系统可能负性调节DO的过程。
Abstract Sympathetic nerve system has been proved to have important regulative effects to bone mass. However, the role of sympathetic nerve system in distraction osteogenesis (DO) is unclear. Here we show that the sympathetic nerve system plays an important role in mandibular DO. Thirty male Sprague-Dawley rats were divided into 2 groups at random. Right-side mandibular DO was performed on the 15 rats in control group (group A). Bilateral transection of cervical sympathetic trunk and right-side mandibular DO were performed on the 15 rats in the experimental group (group B). After operation, quantitative general observations, micro–computed tomography bone morphology analysis, and hematoxylin-eosin staining osseous tissue on new osteotylus in distraction gap were performed at consolidation time of 1, 14, and 28 days. SPSS 12.0 software package was used for statistical analysis. At 1 and 14 days of consolidation time, there was more continuous bone formation in the experimental group than that of the control group as determined by gross observation. Bone formation parameters including bone mineral density, bone volume–total volume ratio, bone trabeculae number as determined by micro-CT, and histological study of the test group were significantly higher than those of the control group (P < 0.05). No significant difference was noted between the 2 groups on consolidation time of 28 days. Our study suggested that the sympathetic innervation loss could improve mandibular DO and new bone formation, and the sympathetic nerve system might negatively regulate the process of DO.