Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae
Morphogenetic pathway of spore wall assembly in Saccharomyces cerevisiae
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DOI：
10.1128/ec.3.6.1464-1475.2004
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发表时间：
2004-12-01
期刊：
影响因子：
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通讯作者：
Neiman, AM
中科院分区：
文献类型：
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作者：
Coluccio, A;Bogengruber, E;Neiman, AM
酿酒酵母孢子通过多层细胞外基质（孢子壁）保护免受环境损害，所述孢子壁在孢子形成期间重新组装。在筛选引起孢子对乙醚蒸气敏感的突变时，鉴定出一组孢子壁组装缺陷的突变体。在这些突变体中的10个的孢子壁缺陷已在各种细胞学和生物化学测定的特点。许多个别突变体在孢子壁内的特定层的组装中有缺陷，导致在组装的离散阶段停滞。这些基因产物中的几个的定位已经确定，并且区分可能直接参与孢子壁组装的蛋白质和可能的调节蛋白质。结果表明，孢子壁的建设涉及一系列依赖的步骤，并提供了一个复杂的细胞外结构的组装形态发生途径的轮廓。
The Saccharomyces cerevisiae spore is protected from environmental damage by a multilaminar extracellular matrix, the spore wall, which is assembled de novo during spore formation. A set of mutants defective in spore wall assembly were identified in a screen for mutations causing sensitivity of spores to ether vapor. The spore wall defects in 10 of these mutants have been characterized in a variety of cytological and biochemical assays. Many of the individual mutants are defective in the assembly of specific layers within the spore wall, leading to arrests at discrete stages of assembly. The localization of several of these gene products has been determined and distinguishes between proteins that likely are involved directly in spore wall assembly and probable regulatory proteins. The results demonstrate that spore wall construction involves a series of dependent steps and provide the outline of a morphogenetic pathway for assembly of a complex extracellular structure.