Penetration and activation of brain endothelium by Salmonella enterica serovar Typhimurium.
Penetration and activation of brain endothelium by Salmonella enterica serovar Typhimurium.
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DOI：
10.1093/infdis/jiq048
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
N. V. van Sorge;Patricia Zialcita;S. Browne;Darin F. Quach;D. Guiney;K. Doran
中科院分区：
文献类型：
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作者：
N. V. van Sorge;Patricia Zialcita;S. Browne;Darin F. Quach;D. Guiney;K. Doran
沙门氏菌脑膜炎是一种严重的中枢神经系统疾病，在非洲尤其常见。在这里，我们表明，沙门氏菌肠道血清型鼠伤寒沙门氏菌能够粘附，侵入，并穿透人脑微血管内皮细胞（hBMEC），构成血脑屏障（BBB）的单细胞层。细胞侵袭依赖于宿主肌动蛋白细胞骨架重排，而功能性III型分泌系统的表达不是必需的。此外，沙门氏菌感染激活了针对中性粒细胞信号传导和募集的促炎免疫应答。沙门氏菌的入侵和免疫激活可能是穿透血脑屏障和沙门氏菌脑膜炎发展的关键步骤。
Salmonella meningitis is a serious disease of the central nervous system, common particularly in Africa. Here, we show that Salmonella enterica serovar Typhimurium is able to adhere, invade, and penetrate human brain microvascular endothelial cells (hBMECs), the single-cell layer constituting the blood-brain barrier (BBB). Cellular invasion was dependent on host actin cytoskeleton rearrangements, while expression of a functional type III secretion system was not essential. In addition, Salmonella infection activated a proinflammatory immune response targeting neutrophil signaling and recruitment. Salmonella invasion and immune activation may represent a crucial step in the penetration of the BBB and development of Salmonella meningitis.