Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis
Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis
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DOI：
10.1016/j.phytol.2019.03.002
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发表时间：
2019-06-01
影响因子：
1.7
通讯作者：
Staerk, Dan
中科院分区：
文献类型：
--
作者：
Chu, Chu;Li, Tuo;Staerk, Dan
2型糖尿病是一种慢性代谢紊乱，影响着全球数百万人，迫切需要新的药物先导。铁皮石斛的干茎在中国被用于缓解糖尿病的症状，但对其生物活性成分的研究有限。我们采用了一种简单、快速、有效的方法，将自由基清除、α-葡萄糖苷酶和α-淀粉酶抑制的三重高分辨率分析与高效液相色谱-光电二极管阵列检测-高分辨率质谱-固相萃取-核磁共振光谱（HR-生物测定/HPLC-PDA-HRMS-SPE-NMR）相结合。鉴定了两个新化合物：3，4，4 ′-三羟基-5-甲氧基联苄（5）和3，4-二羟基-3 ′，4 ′，5-三甲氧基联苄（8a），沿着鉴定了14个已知化合物。其中12个与自由基清除活性相关，6个与α-葡萄糖苷酶抑制相关，1个与α-淀粉酶抑制相关。首次报道了化合物5、树烛素U和3，4-二羟基-4 ′，5-二甲氧基联苄基（12）作为α-葡糖苷酶抑制剂，并且首次报道了12作为α-淀粉酶抑制剂。
Type 2 diabetes is a chronic metabolic disorder affecting millions of people worldwide, and new drug leads are urgently needed. The dried stem of Dendrobium officinale is used in China to alleviate the symptoms of diabetes, but investigations of its bioactive components have been limited. We have applied a simple, fast, and effective method, combining triple high-resolution profiling of radical scavenging and alpha-glucosidase and alpha-amylase inhibition with high-performance liquid chromatography - photodiode-array detection - high-resolution mass spectrometry - solid-phase extraction - nuclear magnetic resonance spectroscopy (HR-bioassay/HPLC-PDA-HRMS-SPE-NMR). Two new compounds, 3,4,4'-trihydroxy-5-methoxybibenzyl (5) and 3,4-dihydroxy-3',4',5-trimethoxybibenzyl (8a), along with fourteen known compounds, were identified. Twelve of these were associated with radical scavenging activity, six with alpha-glucosidase inhibition, and one with a-amylase inhibition. Compound 5, dendrocandin U, and 3,4-dihydroxy-4',5-dimethoxybibenzyl (12) are reported as alpha-glucosidase inhibitors for the first time, and 12 is furthermore reported as an alpha-amylase inhibitor for the first time.