Effect of Protective Coating on Surface Properties and Candida albicans Adhesion to Denture Base Materials
Effect of Protective Coating on Surface Properties and Candida albicans Adhesion to Denture Base Materials
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DOI：
10.1111/jopr.13118
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发表时间：
2019-11-26
影响因子：
4
通讯作者：
Gad, Mohammed M.
中科院分区：
文献类型：
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作者：
AlBin-Ameer, Mohammed Ali;Alsrheed, Mahdi Y.;Gad, Mohammed M.
目的评价防护涂层对口腔基托材料表面粗糙度、接触角及白色念珠菌粘附性的影响。材料与方法制备560个矩形热自聚合丙烯酸树脂样品，按涂覆剂分为5组（n = 14/组）。未涂覆的标本作为对照。涂层材料：纳米涂层、Optiglaze、纳米二氧化硅或氰基丙烯酸酯。使用轮廓仪评估表面粗糙度（R-a）。接触角测量采用固定式滴法进行。采用直接培养法和载玻片计数法评价白色念珠菌粘附性。数据分析采用方差分析和Tukey事后检验（alpha = 0.05）。结果纳米涂层和Optiglaze涂层显著降低了热聚合丙烯酸的R-a （p < 0.001）。纳米二氧化硅涂层对R-a无显著影响，而氰基丙烯酸酯涂层显著增加了R-a （p < 0.001）。对于自聚合丙烯酸，纳米涂层、Optiglaze和纳米二氧化硅涂层与对照组相比显著降低了R-a (p < 0.001)，而对照组和氰基丙烯酸酯涂层之间没有显著差异（p = 0.45）。与对照组相比，纳米涂层、Optiglaze涂层和纳米二氧化硅涂层均显著降低了两种义齿基托材料的接触角和白色念珠菌的粘附（p < 0.001），而氰基丙烯酸酯显著增加了白色念珠菌的粘附（p = 0.002），对热聚树脂（p = 1.00）和自聚合树脂（p = 0.83）的接触角没有影响。结论纳米涂层、Optiglaze或纳米二氧化硅涂层是降低白色念珠菌粘附的有效方法。
Purpose To evaluate the effect of protective coatings on the surface roughness, contact angle, and Candida albicans (C. albicans) adhesion to polymethyl methacrylate (PMMA) denture base materials. Material and Methods A total of 560 rectangular heat- and autopolymerized acrylic resin specimens were fabricated and divided into 5 groups (n = 14/group) according to coating agent. Uncoated specimens were used as control. Coating materials were: nano-coat, Optiglaze, nano-silica, or cyanoacrylate. Surface roughness (R-a) was evaluated using a profilometer. Contact angle measurements were performed using the sessile drop method. C. albicans adhesion was evaluated using direct culture and slide count methods. ANOVA and Tukey's post hoc tests were used for data analysis (alpha = 0.05). Results Nano-coat and Optiglaze coating of heat-polymerized acrylic significantly decreased R-a (p < 0.001). No significant effect was seen with nano-silica coating while R-a significantly increased with cyanoacrylate (p < 0.001). For autopolymerized acrylic, nano-coat, Optiglaze, and nano-silica coatings significantly decreased R-a in comparison to control group (p < 0.001) while no significant difference was seen between control and cyanoacrylate coating (p = 0.45). In comparison to control group, nano-coat, Optiglaze, and nano-silica coating for both denture base materials significantly decreased contact angle, and C. albicans adhesion (p < 0.001) while cyanoacrylate significantly increased C. albicans adhesion (p = 0.002) with no effect on contact angle for heat- (p = 1.00) and autopolymerized resins (p = 0.83). Conclusion Coating of removable prosthesis with nano-coat, Optiglaze, or nano-silica is an effective method to reduce C. albicans adhesion.