Survival of intracellular freezing by the Antarctic nematode Panagrolaimus davidi
Survival of intracellular freezing by the Antarctic nematode Panagrolaimus davidi
复制标题
南极线虫 Panagrolaimus davidi 细胞内冷冻的存活
DOI：
--
复制
发表时间：
1995
影响因子：
2.8
通讯作者：
Ferns
中科院分区：
文献类型：
--
作者：
Wharton;Ferns
通常认为，只有当冰被限制在体腔和细胞外空间时，动物才能在体内的冰形成中幸存下来。细胞内结冰被认为是致命的。这一结论是基于对哺乳动物细胞超低温保存的研究。在一些活的昆虫细胞中观察到了细胞内冻结，但在完整的动物中没有观察到。线虫是透明的，因此可以使用冷冻显微镜直接观察它们体内的冰的位置。我们观察到南极线虫Panagrolaimus Davidi的所有身体隔室，包括细胞内隔室都冻结和融化。来自周围水的灌注性冻结通过身体开口发生，而不是穿过角质层；最常见的是通过排泄孔发生。冻结在细胞内的个别线虫随后将在培养中生长和繁殖。确定这种线虫在细胞内冷冻后存活的机制可能在各种生物材料的低温保存中有重要的应用。
Animals are usually thought to survive ice formation in their bodies only if the ice is confined to the body cavity and to extracellular spaces. Intracellular ice formation is believed to be fatal. This conclusion is based on studies of the cryopreservation of mammalian cells. Intracellular freezing has been observed in some living insect cells but has not been observed in intact animals. Nematodes are transparent and so the location of ice in their bodies can be observed directly using a cryomicroscope stage. We have observed freezing and melting in all body compartments, including intracellular compartments, of the Antarctic nematode Panagrolaimus davidi. Inoculative freezing from the surrounding water occurs via the body openings, rather than across the cuticle; most frequently it occurs via the excretory pore. Individual nematodes that have frozen intracellularly will subsequently grow and reproduce in culture. Determining the mechanisms by which this nematode survives intracellular freezing could have important applications in the cryopreservation of a variety of biological materials.