Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity
Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity
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DOI：
10.1016/j.jacc.2007.08.033
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发表时间：
2007-12-04
影响因子：
24
通讯作者：
Peterson, Eric D.
中科院分区：
文献类型：
--
作者：
Povsic, Thomas J.;Zavodni, Katherine L.;Peterson, Eric D.
目的我们的目标是开发和评估一种新的内源性祖细胞（EPC）检测的基础上醛脱氢酶（ALDH）的活性，并确定ALDH明亮（ALDH br）细胞与以前定义的EPCs，患者年龄，冠状动脉diseases.Background的关系循环EPCs的准确评估是显着的利益，但目前的检测方法有局限性。祖细胞显示高水平的ALDH活性。ALDH活性的基础上进行的测定可以提供一个简单的手段枚举EPCs.Methods我们同时确定EPCs的数量ALDH活性和细胞表面表达的CD 133，CD 34，血管内皮生长因子受体-2的基础上：110例接受心导管检查。我们评估了这些估计的可重复性，EPC检测之间的相关性，以及ALDH br数与年龄和疾病severity.Results的关联乙醛脱氢酶明亮的细胞很容易确定在非动员外周血中的中位数和平均频率为0.041%和0.074%，分别。醛脱氢酶-亮细胞表达CD 34或CD 133细胞表面标记物（分别为57.0%和27.1%），与CD 133 'CD 34'细胞密切相关（r = 0.72; p < 0.001），并且比CD 133（+）CD 34（+）细胞更有效地分化为内皮细胞。乙醛脱氢酶bright细胞数量与患者年龄和冠状动脉疾病severity.Conclusions呈负相关乙醛脱氢酶活性是一种新的简化的方法定量EPCs。ALDH br细胞与临床因素和结果的相关性值得进一步研究。
Objectives Our objective was to develop and assess a novel endogenous progenitor cell (EPC) assay based on aldehyde dehydrogenase (ALDH) activity, and to define the relationship of ALDH-bright (ALDH br) cells with previously defined EPCs, patient age, and extent of coronary artery disease.Background Accurate assessment of circulating EPCs is of significant interest, yet current assays have limitations. Progenitor cells display high levels of ALDH activity. An assay based on ALDH activity may offer a simple means for enumerating EPCs.Methods We simultaneously determined the numbers of EPCs based on ALDH activity and cell surface expression of CD133, CD34, and vascular endothelial growth factor receptor-2 in :110 patients undergoing cardiac catheterization. We assessed the reproducibility of these estimates, correlation among EPC assays, and the association of ALDH br numbers with age and disease severity.Results Aldehyde dehydrogenase-bright cells were easily identified in nonmobilized peripheral blood with median and mean frequencies of 0.041% and 0.074%, respectively. Aldehyde dehydrogenase-bright cells expressed CD34 or CD133 cell surface markers (57.0% and 27.1%, respectively), correlated closely with CD133'CD34' cells (r = 0.72; p < 0.001), and differentiated into endothelial cells with greater efficiency than CD133(+)CD34(+) cells. Aldehyde dehydrogenasebright cell numbers were inversely associated with patient age and coronary disease severity.Conclusions Aldehyde dehydrogenase activity represents a novel simplified method for quantifying EPCs. The correlation of ALDH br cells with clinical factors and outcomes warrants further study.