The involvement of a P38-like MAP kinase in ABA-induced and H2O2-mediated stomatal closure in Vicia faba L
The involvement of a P38-like MAP kinase in ABA-induced and H2O2-mediated stomatal closure in Vicia faba L
复制标题
P38 样 MAP 激酶参与 ABA 诱导和 H2O2 介导的蚕豆气孔关闭。
DOI：
10.1007/s00299-007-0449-x
复制
发表时间：
2008-02-01
影响因子：
6.2
通讯作者：
Song, Chun-Peng
中科院分区：
文献类型：
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作者：
Jiang, Jing;Wang, Pengtao;Song, Chun-Peng
SB203580是p38丝裂原活化蛋白（MAP）激酶的特异性抑制剂，已被广泛用于研究p38在动物和酵母细胞中的生理作用。本文采用表皮条生物测定、激光扫描共聚焦显微镜和全细胞膜片钳技术，研究了吡啶基咪唑类化合物SB 203580对蚕豆保卫细胞H2O2信号转导的影响。结果表明，SB203580能阻断阿坝和H2O2诱导的气孔关闭、ABA诱导的H2O2产生以及K+跨膜通量的降低，而SB202474对这些事件无影响。因此，这些结果表明，激活p38样MAP激酶调节保卫细胞的ROS信号在应激反应。
SB203580 is a specific inhibitor of p38 mitogen-activated protein (MAP) kinase and has been widely used to investigate the physiological roles of p38 in animal and yeast cells. Here by using an epidermal strip bioassay, laser-scanning confocal microscopy and whole-cell patch clamp analysis, we assess the effects of pyridinyl imidazoles-like SB203580 on the H2O2 signaling in guard cells of Vicia faba L. The results indicated that SB203580 blocks H2O2- or ABA-induced stomatal closure, ABA-induced H2O2 generation, and decrease in K+ fluxing across plasma membrane of Vicia guard cells by application of ABA and H2O2, whereas its analog SB202474 had no effect on these events. Thus, these results suggest that activation of p38-like MAP kinase modulates guard cell ROS signaling in response to stress.