Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps
Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps
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DOI：
10.1186/gb-2010-11-4-r41
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发表时间：
2010-01-01
期刊：
影响因子：
12.3
通讯作者：
Berriman, Matthew
中科院分区：
文献类型：
--
作者：
Tsai, Isheng J.;Otto, Thomas D.;Berriman, Matthew
测序技术的进步使得基因组测序的成本大大降低。然而，汇集的数据往往是高度分散的，有许多空白。我们提出了一种实用的方法，使用Illumina序列，以改善草案基因组组装序列对重叠群末端和进行本地组装，以产生跨越缺口的重叠群。因此，基因组草图的连续性可以大大提高，通常不需要产生新的数据。
Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.