Identification of Salt Tolerance-related microRNAs and Their Targets in Maize (Zea mays L.) Using High-throughput Sequencing and Degradome Analysis.
Identification of Salt Tolerance-related microRNAs and Their Targets in Maize (Zea mays L.) Using High-throughput Sequencing and Degradome Analysis.
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DOI：
10.3389/fpls.2017.00864
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Fu R;Zhang M;Zhao Y;He X;Ding C;Wang S;Feng Y;Song X;Li P;Wang B
为了鉴定参与玉米（Zea mays）对盐胁迫的响应和适应的已知和新的microRNAs （miRNAs）及其靶点，通过对小rna （sRNA）和降解文库的深度测序联合分析鉴定了miRNAs及其靶点。通过对超过1亿个原始的sRNA和降解序列进行定量表达分析，确认了这些身份。从4个玉米文库中共鉴定出1040个已知的mirna，其中762个和726个分别来自叶片和根，448个是叶片和根之间共有的mirna。根据相同的标准，共选择了37个潜在的新mirna，以响应盐胁迫。除了已知的miR167和miR164种外，还鉴定了新的推测的miR167和miR164种。miRNA的深度测序和降解体[对其靶标进行定量逆转录聚合酶链反应（qRT-PCR）分析]表明，不止一种新型miRNA可能在玉米对盐度的响应中发挥关键作用。此外，mirna与其靶点之间的相互作用可能在玉米不同部位对盐度的响应中发挥着不同的作用。
To identify the known and novel microRNAs (miRNAs) and their targets that are involved in the response and adaptation of maize (Zea mays) to salt stress, miRNAs and their targets were identified by a combined analysis of the deep sequencing of small RNAs (sRNA) and degradome libraries. The identities were confirmed by a quantitative expression analysis with over 100 million raw reads of sRNA and degradome sequences. A total of 1040 previously known miRNAs were identified from four maize libraries, with 762 and 726 miRNAs derived from leaves and roots, respectively, and 448 miRNAs that were common between the leaves and roots. A total of 37 potential new miRNAs were selected based on the same criteria in response to salt stress. In addition to known miR167 and miR164 species, novel putative miR167 and miR164 species were also identified. Deep sequencing of miRNAs and the degradome [with quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of their targets] showed that more than one species of novel miRNA may play key roles in the response to salinity in maize. Furthermore, the interaction between miRNAs and their targets may play various roles in different parts of maize in response to salinity.