Are serum amyloid A or D-lactate useful to diagnose synovial contamination or sepsis in horses?
Are serum amyloid A or D-lactate useful to diagnose synovial contamination or sepsis in horses?
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DOI：
10.1136/vr.104386
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发表时间：
2017-10-01
影响因子：
2.2
通讯作者：
Rubio-Martinez, Luis M.
中科院分区：
文献类型：
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作者：
Robinson, Claire S.;Singer, Ellen R.;Rubio-Martinez, Luis M.
马滑膜败血症是危及生命的，准确的诊断允许及时治疗是必要的。本研究评估了血清淀粉样蛋白A （SAA）和血液和滑液（SF）中d -乳酸作为马滑膜脓毒症的诊断指标的诊断价值，并将它们与SF中总有核细胞计数（TNCC）、中性粒细胞百分比（%N）和总蛋白（TP）相关联。在一项病例对照观察性研究中，测定了112匹马的血液和SF中SAA和d -乳酸浓度（38匹马滑膜污染或败血症（SCS）， 66匹马滑膜内非败血症病理（nssp）和8匹对照）。SCS的血液和SF SAA显著高于nssp和对照马。nssp和对照马的SAA值相似。SF SAA与滑膜TNCC、TP、血SAA呈正相关。血液和SF SAA诊断SCS的敏感性分别为82.4%和80%，特异性分别为88.9%和73%，临界值分别为60.7和1.14 μ g/ml。各组间血d -乳酸浓度和SF -乳酸浓度无显著差异。本研究表明，血液和SF SAA浓度可以帮助区分SCS与非脓毒性滑膜病理；然而，d -乳酸没有作用。
Synovial sepsis in horses is life threatening and accurate diagnosis allowing prompt treatment is warranted. This study assessed the diagnostic value of serum amyloid A (SAA) and D-lactate in blood and synovial fluid (SF) as diagnostic markers of synovial sepsis in horses and correlated them with total nucleated cell count (TNCC), percentage of neutrophils (%N) and total protein (TP) in SF. Blood and SF SAA and D-lactate concentrations were determined in a case-control observational study including 112 horses (38 with synovial contamination or sepsis (SCS), 66 with non-septic intra-synovial pathology (NSISP) and 8 controls). Blood and SF SAA were significantly higher in SCS than in NSISP and control horses. SAA values were similar in NSISP and control horses. SF SAA was moderately correlated with synovial TNCC, TP and blood SAA. Blood and SF SAA were 82.4 per cent and 80 per cent sensitive and 88.9 per cent and 73 per cent specific for diagnosis of SCS, with cut-off values of 60.7 and 1.14 mu g/ml, respectively. Blood and SF D-lactate concentrations were not significantly different between groups. This study shows that blood and SF SAA concentrations can aid to distinguish SCS from non-septic synovial pathology; however, D-lactate was not useful.