Determination of metacycline and related substances by column liquid chromatography on poly(styrene-divinylbenzene).
Determination of metacycline and related substances by column liquid chromatography on poly(styrene-divinylbenzene).
复制标题
聚苯乙烯-二乙烯基苯柱液相色谱法测定美他环素及有关物质。
DOI：
10.1016/0021-9673(91)80025-c
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
J. Hoogmartens
中科院分区：
文献类型：
--
作者：
Nai;K. Verresen;E. Roets;J. Hoogmartens
Isocratic column liquid chromatography on poly(styrene-divinylbenzene) copolymer allowed complete separation of metacycline, 4-epimetacycline, oxytetracycline, doxycycline and 6-epidoxycycline. 2-Acetyl-2-decarboxamidometacycline was eluted on the tail of metacycline. The mobile phase was 2-methyl-2-propanol-0.2Mphosphate buffer (pH 9.0)-0.01Msodium ethylenediaminetetraacetate (pH 9.0)-water (2.5:10:10:77.5, m/v/v/v). The flow-rate was 1.0 ml/min and detection was performed at 254 nm. Official standards were compared and a number of commercial bulk samples and specialties were analysed. 2-Acetyl-2-decarboxamidometacycline, 6-epidoxy-cycline and doxycycline were the main impurities, while 4-epimetacycline and oxytetracycline were minor impurities.
