Effects of Tranilast and Pentoxifylline in a Mouse Model of Chronic Asthma Using House Dust Mite Antigen
Effects of Tranilast and Pentoxifylline in a Mouse Model of Chronic Asthma Using House Dust Mite Antigen
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DOI：
10.3109/02770900903089998
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发表时间：
2009-01-01
影响因子：
1.9
通讯作者：
Kim, Young Kyoon
中科院分区：
文献类型：
--
作者：
Kim, Seung Joon;Kim, Jin Woo;Kim, Young Kyoon
曲尼司特因其对肥大细胞的抑制作用而被用于过敏性疾病;它在几种疾病中也具有抗纤维化作用。戊茶碱（PTX）是一种甲基黄嘌呤衍生物，是一种有效的抗炎药物，已知其通过抑制Th 1细胞因子而显示其作用，但对Th 2细胞因子的作用不确定。7周龄雌性BALB/c小鼠作为慢性哮喘模型进行研究。用屋尘螨（HDM）抗原攻击小鼠7周。每组小鼠在抗原施用前腹膜内注射曲尼司特、PTX或曲尼司特加PTX。在慢性哮喘小鼠模型中，曲尼司特和PTX均对气道重塑以及气道高反应性（AHR）和气道炎症具有抑制作用。这些药物的改善事件与抑制Th 2细胞因子IL-13和TGF-β 1有关。免疫组织化学分析显示，每个治疗组中支气管周围三色染色面积的减少与支气管周围平滑肌增生、I型胶原和III型胶原沉积的改善相关。这些药物可能对慢性哮喘有潜在的有益作用，特别是在气道重塑方面。
Tranilast has been used in allergic diseases because of its inhibitory effect on mast cells; it also has an anti-fibrotic effect in several diseases. Pentoxifylline (PTX), a methylxanthine derivative, is a potent anti-inflammatory drug that is known to manifest its effect through the inhibition of Th1 cytokine, but with an uncertain effect on Th2 cytokine. Seven-week-old female BALB/c mice were studied as a chronic asthma model. The mice were challenged with house dust mite (HDM) antigen for 7 weeks. Each group of mice was given an intraperitoneal injection of tranilast, PTX, or tranilast plus PTX before antigen administration. In this mouse model of chronic asthma, tranilast, and PTX each had an inhibitory effect on airway remodeling as well as on airway hyperresponsiveness (AHR) and airway inflammation. The improved events of these drugs were related with the inhibition of the Th2 cytokine IL-13 and TGF-beta 1. Immunohistochemical analysis showed that decreases in the peribronchial trichrome stained area in each treatment group were associated with improvements in the peribronchial smooth muscle hyperplasia, collagen type I, and collagen type III deposition. These drugs could have potential beneficial effects on chronic asthma, especially with respect to airway remodeling.