Dynamics of Sept4 expression in fibrotic livers of mice infected with Schistosoma japonicum
Dynamics of Sept4 expression in fibrotic livers of mice infected with Schistosoma japonicum
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日本血吸虫感染小鼠纤维化肝脏中 Sept4 表达动态
DOI：
10.1017/s0031182011000667
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发表时间：
2011-07-01
期刊：
影响因子：
2.4
通讯作者：
Chen, J. L.
中科院分区：
文献类型：
--
作者：
Duan, Y. N.;Qian, H. Y.;Chen, J. L.
摘要：为了探讨血吸虫感染小鼠Septin4（Sept4）的表达动态及其在肝纤维化形成中的作用，我们构建了24周的血吸虫卵诱导肝纤维化小鼠模型。采用免疫组化染色、qRT-PCR和Western blot检测Sept4和α-平滑肌肌动蛋白（α-SMA）的表达。我们发现 Sept4 位于窦周间隙，肝星状细胞 (HSC) 分布在周围卵圆肉芽肿和门静脉的周围。 Sept4和α-SMA的表达具有类似的显着趋势，即在感染后12周（p.i.）上调至峰值，然后下调。注射后 24 周时两者都处于低水平。这些结果表明 Sept4 和 α-SMA 可能在 HSC 中相互作用。基于这一证据，我们假设 Sept4 似乎通过调节 HSC 活化参与炎症肉芽肿的形成和随后的肝纤维化。
SUMMARY In order to investigate the dynamics of Septin4 (Sept4) expression and its function in the formation of fibrotic livers in mice infected with Schistosoma japonicum, we constructed the mouse model of S. japonicum egg-induced liver fibrosis for 24 weeks. Immunohistochemical staining, qRT-PCR and Western blot were used to detect the expression of Sept4 and α-smooth muscle actin (α-SMA). We found Sept4 localized in the perisinusoidal space where hepatic stellate cells (HSCs) distribute in the periphery of circumoval granulomas and the portal venule. The expression of Sept4 and α-SMA had a similar significant tendency of an up-regulation to a peak at 12 weeks post-infection (p.i.) followed by a down-regulation. At 24 weeks p.i. both were at a low level. These results suggest that Sept4 and α-SMA may interact together in HSCs. Based on this evidence, we hypothesize that Sept4 seems to be involved in the formation of inflammatory granulomata and subsequent liver fibrosis by regulating HSCs activation.