TRANSPLANTATION OF CULTURED BOVINE CORNEAL ENDOTHELIAL CELLS TO RABBIT CORNEA - CLINICAL IMPLICATIONS FOR HUMAN STUDIES
TRANSPLANTATION OF CULTURED BOVINE CORNEAL ENDOTHELIAL CELLS TO RABBIT CORNEA - CLINICAL IMPLICATIONS FOR HUMAN STUDIES
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DOI：
10.1073/pnas.76.1.464
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
ALVARADO, J
中科院分区：
文献类型：
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作者：
GOSPODAROWICZ, D;GREENBURG, G;ALVARADO, J
用牛角膜内皮细胞（来自阉牛）包被剥除内皮的兔角膜，该细胞先前在组织培养中维持了短期（20代）或长期（200代）。当移植回雌性家兔时，角膜按钮保持透明，没有水肿。涂有牛角膜细胞并移植到兔子体内的裸露角膜在7天内变得不透明和水肿，并在此后保持不变。移植角膜的牛角膜内皮细胞保持透明超过100天，当放回组织培养时增殖活跃。移植物的角膜内皮细胞的染色体数目为2n = 60。性染色体为男性特征（XY）。受体兔内皮细胞染色体数目为2n = 44，具有雌性（XX）性染色体特征。核型分析显示，受体内皮细胞没有侵入角膜扣，相反，角膜扣上的内皮细胞也没有侵入受体内皮细胞。培养的角膜内皮细胞在体外保持功能，并可在体内替代受损或无功能的内皮。
Rabbit corneas denuded of their endothelium were coated with bovine corneal endothelial cells (from steers) previously maintained in tissue culture for short (20 generations) or prolonged (200 generations) periods. When grafted back into female rabbits, the corneal buttons remained clear and showed no edema. Denuded corneas coated with bovine keratocytes and grafted into rabbits became opaque and edematous within 7 days and remained so thereafter. Bovine corneal endothelial cells of the grafted corneas, which remained clear for over 100 days, proliferated actively when put back into tissue culture. The corneal endothelial cells of the graft had a chromosome number of 2n = 60. The sex chromosomes were characteristic of the male (XY). The chromosome number of the endothelium of the recipient rabbit was 2n = 44 with sex chromosomes characteristics of the female (XX). Karyotype analysis showed that there was no invasion of the corneal button by the recipient endothelium and, conversely, no invasion of the recipient endothelium by the endothelium on the corneal button. Cultured corneal endothelial cells remain functional in vitro and can replace a damaged or nonfunctional endothelium in vivo.